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Electrochemical biosensor for Mycobacterium tuberculosis DNA detection based on gold nanotubes array electrode platform

Electrochemical biosensor for Mycobacterium tuberculosis DNA detection based on gold nanotubes array electrode platform
Torati, R[Torati, Ramulu]Reddy, V[Reddy, Venu]Yoon, SS[Yoon, Seok Soo]Kim, C[Kim, CheolGi]
DGIST Authors
Torati, R[Torati, Ramulu]Reddy, V[Reddy, Venu]Kim, C[Kim, CheolGi]
Issued Date
Article Type
Aromatic CompoundsBacterial DNABiosensing TechniquesBiosensorBiosensorsChemistryComplementary DnaCyclic PotentiometryCyclic VoltammetryDNADNA BiosensorDNA BiosensorsDNA DeterminationDNA HybridizationDNA ProbeDNA, BacterialElectro-Chemical ElectrodesElectrochemical AnalysisElectrochemical BiosensorElectrochemical DepositionElectrochemical DNA BiosensorsElectrochemical PerformanceElectrochemical TechniquesElectrodepositionElectrodesElectron TransportGenetic ProceduresGoldGold NanotubeGold Nanotube ArrayHumanHumansHybridizationIsolation and PurificationLimit of DetectionMethylene BlueMicrobiologyMicroscopy, Electron, ScanningMycobacterium TuberculosisNanoarrayNanofabricationNanotubeNanotubesNon-HumanPathogenicityReductionScanning Electron MicroscopySynthesis (Chemical)Thick FilmsTuberculosisUnclassified DrugYarn
The template assisted electrochemical deposition technique was used for the synthesis of gold nanotubes array (AuNTsA). The morphological structure of the synthesized AuNTsA was observed by scanning electron microscopy and found that the individual nanotubes are around 1.5μm in length with a diameter of 200nm. Nanotubes are vertically aligned to the Au thick film, which is formed during the synthesis process of nanotubes. The electrochemical performance of the AuNTsA was compared with the bare Au electrode and found that AuNTsA has better electron transfer surface than bare Au electrode which is due to the high surface area. Hence, the AuNTsA was used as an electrode for the fabrication of DNA hybridization biosensor for detection of Mycobacterium Tuberculosis DNA. The DNA hybridization biosensor constructed by AuNTsA electrode was characterized by cyclic voltammetry technique with Fe(CN)63-/4- as an electrochemical redox indicator. The selectivity of the fabricated biosensor was illustrated by hybridization with complementary DNA and non-complementary DNA with probe DNA immobilized AuNTsA electrode using methylene blue as a hybridization indicator. The developed electrochemical DNA biosensor shows good linear range of complementary DNA concentration from 0.01ng/μL to 100ng/μL with high detection limit. © 2015 Elsevier B.V.
Related Researcher
  • 김철기 Kim, CheolGi 화학물리학과
  • Research Interests Magnetic Materials and Spintronics; Converging Technology of Nanomaterials and Biomaterials; Bio-NEMS;MEMS
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Department of Physics and Chemistry Lab for NanoBio-Materials & SpinTronics(nBEST) 1. Journal Articles
Department of Physics and Chemistry ETC 1. Journal Articles


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