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Feeding State, Insulin and NPR-1 Modulate Chemoreceptor Gene Expression via Integration of Sensory and Circuit Inputs
- Feeding State, Insulin and NPR-1 Modulate Chemoreceptor Gene Expression via Integration of Sensory and Circuit Inputs
- Gruner, Matthew; Nelson, Dru; Winbush, Ari; Hintz, Rebecca; Ryu, Leesun; Chung, Samuel H.; Kim, Kyuhyung; Gabel, Chrisopher V.; van der Linden, Alexander M.
- DGIST Authors
- Kim, Kyuhyung
- Issue Date
- PLoS Genetics, 10(10)
- Article Type
- Animal; Animal Experiment; Animal Tissue; Animals; Animals, Genetically Modified; Biosynthesis; Caenorhabditis Elegans; Caenorhabditis Elegans Protein; Caenorhabditis Elegans Proteins; Calcium Signaling; Controlled Study; DAF-2 Protein; DAF-2 Protein, C. Elegans; Down-Regulation; Feeding; Feeding Behavior; Food Availability; Gap Junction; Gene; Gene Expression Regulation; Genetics; Insulin; Insulin Receptor; Interneuron; Kin-29 Protein; Kin-29 Protein, C. Elegans; MEF-2 Protein, C. Elegans; Metabolism; Mutation; Myocyte Enhancer Factor 2; Neuropeptide Receptor; Neuropeptide Y Receptor; Non-Human; NPR-1 Protein, C. Elegans; NPR 1 Protein; OCR 2 Protein; Physiology; Protein-Serine-Threonine Kinases; Protein Kinase; Protein Serine Threonine Kinase; Receptor, Insulin; Receptors, Neuropeptide Y; Sensory Nerve Cell; Sensory Receptor Cells; Signal Transduction; Srh 234 Gene; Starvation; Transcription Factor; Transcription Factor Daf16; Transcription Factors; Transgenic Animal; Unclassified Drug; Vanilloid Receptor
- Feeding state and food availability can dramatically alter an animals' sensory response to chemicals in its environment. Dynamic changes in the expression of chemoreceptor genes may underlie some of these food and state-dependent changes in chemosensory behavior, but the mechanisms underlying these expression changes are unknown. Here, we identified a KIN-29 (SIK)-dependent chemoreceptor, srh-234, in C. elegans whose expression in the ADL sensory neuron type is regulated by integration of sensory and internal feeding state signals. We show that in addition to KIN-29, signaling is mediated by the DAF-2 insulin-like receptor, OCR-2 TRPV channel, and NPR-1 neuropeptide receptor. Cell-specific rescue experiments suggest that DAF-2 and OCR-2 act in ADL, while NPR-1 acts in the RMG interneurons. NPR-1-mediated regulation of srh-234 is dependent on gap-junctions, implying that circuit inputs regulate the expression of chemoreceptor genes in sensory neurons. Using physical and genetic manipulation of ADL neurons, we show that sensory inputs from food presence and ADL neural output regulate srh-234 expression. While KIN-29 and DAF-2 act primarily via the MEF-2 (MEF2) and DAF-16 (FOXO) transcription factors to regulate srh-234 expression in ADL neurons, OCR-2 and NPR-1 likely act via a calcium-dependent but MEF-2- and DAF-16-independent pathway. Together, our results suggest that sensory- and circuit-mediated regulation of chemoreceptor genes via multiple pathways may allow animals to precisely regulate and fine-tune their chemosensory responses as a function of internal and external conditions. © 2014 Gruner et al.
- Public Library of Science
- Related Researcher
Kim, Kyu Hyung
The K. Kim Lab of Neurobehavior and Neural Circuits
Neurobehavior; Neural Circuit; Neurodevelopment; 신경회로; 신경행동; 신경발생; 신경유전학
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- Department of Brain and Cognitive SciencesThe K. Kim Lab of Neurobehavior and Neural Circuits1. Journal Articles
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