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Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by gamma-irradiation (Co-60) of adventitious roots

Title
Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by gamma-irradiation (Co-60) of adventitious roots
Author(s)
Zhang, Jun-YingSun, Hyeon-JinSong, In-JaBae, Tae-WoongKang, Hong-GyuKo, Suk-MinKwon, Yong-IkKim, Il-WoungLee, JaechunPark, Shin-YoungLim, Pyung OkKim, Yong HwanLee, Hyo-Yeon
Issued Date
2014-07
Citation
Journal of Ginseng Research, v.38, no.3, pp.220 - 225
Type
Article
Author Keywords
Mutant adventitious rootsPanax ginseng MeyerSomatic embryoSomatic embryogenesis
Keywords
SOMATIC EMBRYOGENESISCA-MEYERRAPID MULTIPLICATIONCOTYLEDON EXPLANTSHIGH-FREQUENCYCELL-CULTUREEMBRYOSACCUMULATIONSAPONINCALLUS
ISSN
1226-8453
Abstract
An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. © 2014, The Korean Society of Ginseng, Published by Elsevier. All rights reserved.
URI
http://hdl.handle.net/20.500.11750/3078
DOI
10.1016/j.jgr.2014.04.001
Publisher
Korean Society of Ginseng
Related Researcher
  • 임평옥 Lim, Pyung Ok
  • Research Interests Aging and programmen cell death in plants; RNA-based regulation of leaf aging; Synthetic genetics-based aging reprogramming
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Department of New Biology Plant Senescence Laboratory 1. Journal Articles

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