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Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by gamma-irradiation (Co-60) of adventitious roots

Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by gamma-irradiation (Co-60) of adventitious roots
Zhang, JY[Zhang, Jun-Ying]Sun, HJ[Sun, Hyeon-Jin]Song, IJ[Song, In-Ja]Bae, TW[Bae, Tae-Woong]Kang, HG[Kang, Hong-Gyu]Ko, SM[Ko, Suk-Min]Kwon, YI[Kwon, Yong-Ik]Kim, IW[Kim, Il-Woung]Lee, J[Lee, Jaechun]Park, SY[Park, Shin-Young]Lim, PO[Lim, Pyung-Ok]Kim, YH[Kim, Yong Hwan]Lee, HY[Lee, Hyo-Yeon]
DGIST Authors
Lim, PO[Lim, Pyung-Ok]
Issued Date
Article Type
2,4 Dichlorophenoxyacetic AcidAdventitious RootCallus (Plant)Cell ProliferationCobalt 60Embryo DevelopmentExplantGamma IrradiationGerminationGibberellic AcidGinsengKinetinMutantMutant Adventitious RootsNon-HumanPanax Ginseng MeyerPlant RegenerationPriority JournalShootSoilSomatic EmbryoSomatic EmbryogenesisWild Type
An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants. © 2014, The Korean Society of Ginseng, Published by Elsevier. All rights reserved.
Korean Society of Ginseng
Related Researcher
  • 임평옥 Lim, Pyung Ok
  • Research Interests Aging and programmen cell death in plants; RNA-based regulation of leaf aging; Synthetic genetics-based aging reprogramming
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Department of New Biology CBRG(Complex Biology Research Group) 1. Journal Articles


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