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Erythropoietin promotes hair shaft growth in cultured human hair follicles and modulates hair growth in mice

Erythropoietin promotes hair shaft growth in cultured human hair follicles and modulates hair growth in mice
Kang, BM[Kang, Bo Mi]Shin, SH[Shin, Seung Hyun]Kwack, MH[Kwack, Mi Hee]Shin, H[Shin, HyeRim]Oh, JW[Oh, Ji Won]Kim, J[Kim, Jinoh]Moon, C[Moon, Chanil]Moon, C[Moon, Cheil]Kim, JC[Kim, Jung Chul]Kim, MK[Kim, Moon Kyu]Sung, YK[Sung, Young Kwan]
DGIST Authors
Moon, C[Moon, Cheil]
Issued Date
Article Type
3 (4,5 Dimethyl 2 Thiazolyl) 2,5 Diphenyltetrazolium BromideAgaroseAnimalsC57Bl 6 MouseCell CultureCell ProliferationCell SurvivalCell ViabilityCells, CulturedControlled StudyDermal PapillaDermal Papilla CellEPOEPORErythropoietinFemaleHairHair CellHair CycleHair FollicleHair GrowthHumanHuman CellHuman TissueHumansImmunoblottingIn Vivo StudyKeratinocyteKeratinocytesKi 67 AntigenMaleMessenger RNAMiceMice, Inbred C57BLModels, AnimalMouseNon-HumanPhosphorylationPriority JournalProtein ExpressionProtein Kinase BProtein PhosphorylationProto-Oncogene Proteins C-AKTReceptors, ErythropoietinReverse Transcriptase-Polymerase Chain ReactionRNA, MessengerScalpSignal Transduction
Background: Recent studies have shown that erythropoietin (EPO)/erythropoietin receptor (EPOR) signaling exist in both human and mouse hair follicles (HFs). Objective: To investigate whether dermal papilla cells (DPCs) express functional EPOR and, if so, to investigate effects of EPO on hair shaft growth in cultured human scalp hair follicles and hair growth in mice. Methods: EPOR expression in DPCs and follicular keratinocytes was examined by RT-PCR and immunoblot. Phosphorylation of EPOR signaling pathway mediators by EPO treatment was examined by immunoblot. MTT assay was employed to check cell viability after EPO treatment. Hair shaft growth was measured in the absence or presence of EPO and matrix keratinocyte proliferation was examined by Ki-67 immunostaining in cultured hair follicles. Agarose beads containing EPO were implanted into dorsal skin of C57BL/6 mice to examine effects of EPO on hair growth in vivo. Results: EPOR mRNA and protein are expressed in cultured human DPCs. EPOR signaling pathway mediators such as EPOR and Akt are phosphorylated by EPO in DPCs. EPO significantly promoted the growth of DPCs and elongated hair shafts with increased proliferation of matrix keratinocytes in cultured human hair follicles. In addition, EPO not only promoted anagen induction from telogen but also prolonged anagen phase. Conclusions: EPO may modulate hair growth by stimulating DPCs that express functional EPOR. © 2010 Japanese Society for Investigative Dermatology.
Related Researcher
  • 문제일 Moon, Cheil 뇌과학과
  • Research Interests Brain convergent science based on chemical senses; olfaction; 감각신경계 기반 뇌융합과학; 후각 신경계
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Department of Brain Sciences Laboratory of Chemical Senses 1. Journal Articles


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