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Detection of mutant p53 using field-effect transistor biosensor
- Detection of mutant p53 using field-effect transistor biosensor
- Han, Sang Hee; Kim, Sang Kyu; Park, Kyoungsook; Yi, So Yeon; Park, Hye-Jung; Lyu, Hong-Kun; Kim, Moonil; Chung, Bong Hyun
- DGIST Authors
- Park, Hye-Jung
- Issue Date
- Analytica Chimica Acta, 665(1), 79-83
- Article Type
- Biochemistry; Biosensing Techniques; Biosensor; Biosensors; Controlled Study; DNA; DNA-Binding; DNA-Binding Domain; DNA-Protein Interaction; Drain Current; FET-Type Biosensors; Field-Effect; Field Effect Transistor; Field Effect Transistors; Gene Mutation; Genes; Mesfet Devices; Metal Oxide Semiconductor Field-Effect Transistor; Metal Oxide Semiconductor Field-Effect Transistors; Metallic Compounds; Monitoring; MOS-FET; MOS Devices; Mutagenesis, Site-Directed; Mutant P53; Mutant Protein; Mutation; P53; P53 Protein; Priority Journal; Protein Binding; Protein DNA Interaction; Protein Immobilization; Protein P53; Protein Structure, Tertiary; Proteins; Recombinant Proteins; Semiconductor Analyzer; Sensing Layers; Surface Plasmon Resonance; Surface Property; Transistors, Electronic; Tumor Suppressor Protein P53
- We assessed the abilities of wild p53 and mutant p53 proteins to interact with the consensus DNA-binding sequence using a MOSFET biosensor. This is the first report in which mutant p53 has been detected on the basis of DNA-protein interaction using a FET-type biosensor. In an effort to evaluate the performance of this protocol, we constructed the core domain of wild p53 and mutant p53 (R248W), which is DNA-binding-defective. After the immobilization of the cognate DNA to the sensing layer, wild p53 and mutant p53 were applied to the DNA-coated gate surface, and subsequently analyzed using a semiconductor analyzer. As a consequence, a significant up-shift in drain current was noted in response to wild p53, but not mutant p53, thereby indicating that sequence-specific DNA-protein interactions could be successfully monitored using a field-effect-based biosensor. These data also corresponded to the results obtained using surface plasmon resonance (SPR) measurements. Taken together, our results show that a FET-type biosensor might be promising for the monitoring of mutant p53 on the basis of its DNA-binding activity, providing us with very valuable insights into the monitoring for diseases, particularly those associated with DNA-protein binding events. © 2010 Elsevier B.V.
- Elsevier B.V.
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