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Increased Excitatory Synaptic Transmission of Dentate Granule Neurons in Mice Lacking PSD-95-Interacting Adhesion Molecule Neph2/Kirrel3 during the Early Postnatal Period

Increased Excitatory Synaptic Transmission of Dentate Granule Neurons in Mice Lacking PSD-95-Interacting Adhesion Molecule Neph2/Kirrel3 during the Early Postnatal Period
Roh, Junyeop D.Choi, Su-YeonCho, Yi SulChoi, Tae-YongPark, Jong-SilCutforth, TylerChung, WoosukPark, HanwoolLee, DongsooKim, Myeong-HeuiLee, YeunkumMo, SeojungRhee, Jeong-SeopKim, HyunKo, JaewonChoi, Se-YoungBae, Yong ChulShen, KangKim, EunjoonHan, Kihoon
DGIST Authors
Ko, Jaewon
Issue Date
Frontiers in Molecular Neuroscience, 10
Article Type
Dentate Granule NeuronDentate Granule NeuronExcitatory SynapseExcitatory SynapseIntellectual DisabilityKirrel3Kirrel3LAR RPTPsMutationsNeph2NeuroliginsProtein CASKPSD 95Rat BrainReceptorSpecificitySynapses
Copy number variants and point mutations of NEPH2 (also called KIRREL3) gene encoding an immunoglobulin (Ig) superfamily adhesion molecule have been linked to autism spectrum disorders, intellectual disability and neurocognitive delay associated with Jacobsen syndrome, but the physiological roles of Neph2 in the mammalian brain remain largely unknown. Neph2 is highly expressed in the dentate granule (DG) neurons of the hippocampus and is localized in both dendrites and axons. It was recently shown that Neph2 is required for the formation of mossy fiber filopodia, the axon terminal structure of DG neurons forming synapses with GABAergic neurons of CA3. In contrast, however, it is unknown whether Neph2 also has any roles in the postsynaptic compartments of DG neurons. We here report that, through its C-terminal PDZ domain-binding motif, Neph2 directly interacts with postsynaptic density (PSD)-95, an abundant excitatory postsynaptic scaffolding protein. Moreover, Neph2 protein is detected in the brain PSD fraction and interacts with PSD-95 in synaptosomal lysates. Functionally, loss of Neph2 in mice leads to age-specific defects in the synaptic connectivity of DG neurons. Specifically, Neph2−/− mice show significantly increased spontaneous excitatory synaptic events in DG neurons at postnatal week 2 when the endogenous Neph2 protein expression peaks, but show normal excitatory synaptic transmission at postnatal week 3. The evoked excitatory synaptic transmission and synaptic plasticity of medial perforant pathway (MPP)-DG synapses are also normal in Neph2−/− mice at postnatal week 3, further confirming the age-specific synaptic defects. Together, our results provide some evidence for the postsynaptic function of Neph2 in DG neurons during the early postnatal period, which might be implicated in neurodevelopmental and cognitive disorders caused by NEPH2 mutations. © 2017 Roh, Choi, Cho, Choi, Park, Cutforth, Chung, Park, Lee, Kim, Lee, Mo, Rhee, Kim, Ko, Choi, Bae, Shen, Kim and Han.
Frontiers Research Foundation
Related Researcher
  • Author Ko, Jaewon Laboratory of Synapse Formation and Function
  • Research Interests Synapse Formation and Function; Neural Circuits; 뇌질환; animal model
Department of Brain and Cognitive SciencesLaboratory of Synapse Formation and Function1. Journal Articles

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