Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Choi, Eun-Sook | - |
dc.contributor.author | Lee, Se Geun | - |
dc.contributor.author | Lee, Sung-Jun | - |
dc.contributor.author | Kim, Eunjoo | - |
dc.date.accessioned | 2018-01-25T01:10:47Z | - |
dc.date.available | 2018-01-25T01:10:47Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2015-03 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/5205 | - |
dc.description.abstract | A rapid and easy immunochromatography assay using dye-labeled cellulose nanobeads (CNBs) was developed to detect proteins with hexa-histidine tag (His-tag) to characterize recombinant proteins during purification. Recombinant ATG8 protein was used as a His-tagged protein, and ATG8-conjugated CNBs (A-CNBs) were prepared. The original ATG8 in the sample solution competed with A-CNBs for anti-His-tag antibodies spotted on to the strip resulting in an inverse relationship between ATG8 concentration and the colorimetric signal. The usefulness of this method was shown by adding ATG8 to a 1% Escherichia coli extract. In addition, this assay can be used to detect other His-tagged proteins without protein-specific antibodies. Because the identification of fractions containing His-tagged proteins by western blotting or ELISA is labor-intensive and expensive, our method provides an efficient and cheaper alternative. © 2014, Springer Science+Business Media Dordrecht. | - |
dc.publisher | Springer | - |
dc.title | Rapid detection of 6x-histidine-labeled recombinant proteins by immunochromatography using dye-labeled cellulose nanobeads | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s10529-014-1731-y | - |
dc.identifier.scopusid | 2-s2.0-84925491787 | - |
dc.identifier.bibliographicCitation | Biotechnology Letters, v.37, no.3, pp.627 - 632 | - |
dc.subject.keywordAuthor | Dye-labeled cellulose nanobeads | - |
dc.subject.keywordAuthor | Hexa-histidine tag | - |
dc.subject.keywordAuthor | Immunochromatography | - |
dc.subject.keywordAuthor | Rapid test | - |
dc.subject.keywordAuthor | Recombinant protein detection | - |
dc.citation.endPage | 632 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 627 | - |
dc.citation.title | Biotechnology Letters | - |
dc.citation.volume | 37 | - |
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