Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum
- Title
- Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum
- Author(s)
- Kim, Eunjoo ; Lee, Se Geun ; Kim, Hyun-Chul ; Lee, Sung Jun ; Baek, Chul Su ; Choi, Eun-Sook ; Jeong, Sang Won
- Issued Date
- 2013-11-22
- Citation
- Separation Science and Technology, v.48, no.17, pp.2600 - 2607
- Type
- Article
- Author Keywords
- affinity isolation ; C-reactive protein (crp) ; magnetic nanoparticle ; phosphocholine ; protein purification
- Keywords
- IRON-OXIDE NANOPARTICLES ; AFFINITY-CHROMATOGRAPHY ; SEPARATION ; PURIFICATION ; BEADS ; ACID ; PARTICLES ; LIGANDS
- ISSN
- 0149-6395
- Abstract
- In the present study, we describe the easy isolation of C-reactive protein (CRP) from human serum using phosphocholine-modified magnetic nanoparticles (MNPs). A phosphocholine-based monomer, 3-(4)-vinylbenzyl-12-phosphorylcholine dodecanoate (VPC), was polymerized on methacrylate-coated MNPs and the resulting MNPs (VPC-MNPs) were assessed for their ability to isolate CRP from a human serum sample. CRP could be isolated from human serum with one adsorption step to VPC-MNPs within 1 h. The high purity of the isolated CRP fraction determined by SDS-PAGE indicates a good selectivity of VPC-MNPs for CRP binding. These results demonstrate the feasibility of using ligand-functionalized MNPs for rapid, easy, and efficient protein isolation. © 2013 Copyright Taylor and Francis Group, LLC.
- Publisher
- Taylor and Francis Ltd.
- Related Researcher
-
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Kim, Eunjoo
- Research Interests Biomarker; liquid biopsy; molecular diagnosis; nanobiosensor; drug delivery; exosome
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