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Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum

Title
Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum
Authors
Kim, EunjooLee, Se GeunKim, Hyun-ChulLee, Sung JunBaek, Chul SuChoi, Eun-SookJeong, Sang Won
DGIST Authors
Kim, EunjooLee, Se GeunKim, Hyun-ChulLee, Sung JunJeong, Sang Won
Issue Date
2013-11-22
Citation
Separation Science and Technology, 48(17), 2600-2607
Type
Article
Article Type
Article
Keywords
Affinity IsolationBody FluidsC-Reactive Protein (CRP)C-Reactive ProteinsMagnetic Nano-ParticleMagnetic Nano-ParticlesNanoparticlesPhosphocholineProtein PurificationProteins
ISSN
0149-6395
Abstract
In the present study, we describe the easy isolation of C-reactive protein (CRP) from human serum using phosphocholine-modified magnetic nanoparticles (MNPs). A phosphocholine-based monomer, 3-(4)-vinylbenzyl-12-phosphorylcholine dodecanoate (VPC), was polymerized on methacrylate-coated MNPs and the resulting MNPs (VPC-MNPs) were assessed for their ability to isolate CRP from a human serum sample. CRP could be isolated from human serum with one adsorption step to VPC-MNPs within 1 h. The high purity of the isolated CRP fraction determined by SDS-PAGE indicates a good selectivity of VPC-MNPs for CRP binding. These results demonstrate the feasibility of using ligand-functionalized MNPs for rapid, easy, and efficient protein isolation. © 2013 Copyright Taylor and Francis Group, LLC.
URI
http://hdl.handle.net/20.500.11750/5289
DOI
10.1080/01496395.2013.807516
Publisher
Taylor and Francis Ltd.
Related Researcher
  • Author Kim, Eunjoo  
  • Research Interests Biomarker, liquid biopsy, nanomedicine, molecular diagnosis
Files:
There are no files associated with this item.
Collection:
Magnet-Controlled Materials Research Group1. Journal Articles
Magnet-Controlled Materials Research Group1. Journal Articles


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