Cited time in webofscience Cited time in scopus

Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum

Title
Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum
Author(s)
Kim, EunjooLee, Se GeunKim, Hyun-ChulLee, Sung JunBaek, Chul SuChoi, Eun-SookJeong, Sang Won
Issued Date
2013-11-22
Citation
Separation Science and Technology, v.48, no.17, pp.2600 - 2607
Type
Article
Author Keywords
affinity isolationC-reactive protein (crp)magnetic nanoparticlephosphocholineprotein purification
Keywords
IRON-OXIDE NANOPARTICLESAFFINITY-CHROMATOGRAPHYSEPARATIONPURIFICATIONBEADSACIDPARTICLESLIGANDS
ISSN
0149-6395
Abstract
In the present study, we describe the easy isolation of C-reactive protein (CRP) from human serum using phosphocholine-modified magnetic nanoparticles (MNPs). A phosphocholine-based monomer, 3-(4)-vinylbenzyl-12-phosphorylcholine dodecanoate (VPC), was polymerized on methacrylate-coated MNPs and the resulting MNPs (VPC-MNPs) were assessed for their ability to isolate CRP from a human serum sample. CRP could be isolated from human serum with one adsorption step to VPC-MNPs within 1 h. The high purity of the isolated CRP fraction determined by SDS-PAGE indicates a good selectivity of VPC-MNPs for CRP binding. These results demonstrate the feasibility of using ligand-functionalized MNPs for rapid, easy, and efficient protein isolation. © 2013 Copyright Taylor and Francis Group, LLC.
URI
http://hdl.handle.net/20.500.11750/5289
DOI
10.1080/01496395.2013.807516
Publisher
Taylor and Francis Ltd.
Related Researcher
  • 김은주 Kim, Eunjoo
  • Research Interests Biomarker; liquid biopsy; molecular diagnosis; nanobiosensor; drug delivery; exosome
Files in This Item:

There are no files associated with this item.

Appears in Collections:
Division of Biomedical Technology 1. Journal Articles
Division of AI, Big data and Block chain 1. Journal Articles

qrcode

  • twitter
  • facebook
  • mendeley

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE