Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kim, Eunjoo | - |
dc.contributor.author | Lee, Se Geun | - |
dc.contributor.author | Kim, Hyun-Chul | - |
dc.contributor.author | Lee, Sung Jun | - |
dc.contributor.author | Baek, Chul Su | - |
dc.contributor.author | Choi, Eun-Sook | - |
dc.contributor.author | Jeong, Sang Won | - |
dc.date.accessioned | 2018-01-25T01:12:48Z | - |
dc.date.available | 2018-01-25T01:12:48Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2013-11-22 | - |
dc.identifier.issn | 0149-6395 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/5289 | - |
dc.description.abstract | In the present study, we describe the easy isolation of C-reactive protein (CRP) from human serum using phosphocholine-modified magnetic nanoparticles (MNPs). A phosphocholine-based monomer, 3-(4)-vinylbenzyl-12-phosphorylcholine dodecanoate (VPC), was polymerized on methacrylate-coated MNPs and the resulting MNPs (VPC-MNPs) were assessed for their ability to isolate CRP from a human serum sample. CRP could be isolated from human serum with one adsorption step to VPC-MNPs within 1 h. The high purity of the isolated CRP fraction determined by SDS-PAGE indicates a good selectivity of VPC-MNPs for CRP binding. These results demonstrate the feasibility of using ligand-functionalized MNPs for rapid, easy, and efficient protein isolation. © 2013 Copyright Taylor and Francis Group, LLC. | - |
dc.publisher | Taylor and Francis Ltd. | - |
dc.title | Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum | - |
dc.type | Article | - |
dc.identifier.doi | 10.1080/01496395.2013.807516 | - |
dc.identifier.scopusid | 2-s2.0-84886426070 | - |
dc.identifier.bibliographicCitation | Separation Science and Technology, v.48, no.17, pp.2600 - 2607 | - |
dc.subject.keywordAuthor | affinity isolation | - |
dc.subject.keywordAuthor | C-reactive protein (crp) | - |
dc.subject.keywordAuthor | magnetic nanoparticle | - |
dc.subject.keywordAuthor | phosphocholine | - |
dc.subject.keywordAuthor | protein purification | - |
dc.subject.keywordPlus | IRON-OXIDE NANOPARTICLES | - |
dc.subject.keywordPlus | AFFINITY-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | BEADS | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | PARTICLES | - |
dc.subject.keywordPlus | LIGANDS | - |
dc.citation.endPage | 2607 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 2600 | - |
dc.citation.title | Separation Science and Technology | - |
dc.citation.volume | 48 | - |
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