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dc.contributor.author Kim, Eunjoo -
dc.contributor.author Lee, Se Geun -
dc.contributor.author Kim, Hyun-Chul -
dc.contributor.author Lee, Sung Jun -
dc.contributor.author Baek, Chul Su -
dc.contributor.author Choi, Eun-Sook -
dc.contributor.author Jeong, Sang Won -
dc.date.accessioned 2018-01-25T01:12:48Z -
dc.date.available 2018-01-25T01:12:48Z -
dc.date.created 2017-04-10 -
dc.date.issued 2013-11-22 -
dc.identifier.issn 0149-6395 -
dc.identifier.uri http://hdl.handle.net/20.500.11750/5289 -
dc.description.abstract In the present study, we describe the easy isolation of C-reactive protein (CRP) from human serum using phosphocholine-modified magnetic nanoparticles (MNPs). A phosphocholine-based monomer, 3-(4)-vinylbenzyl-12-phosphorylcholine dodecanoate (VPC), was polymerized on methacrylate-coated MNPs and the resulting MNPs (VPC-MNPs) were assessed for their ability to isolate CRP from a human serum sample. CRP could be isolated from human serum with one adsorption step to VPC-MNPs within 1 h. The high purity of the isolated CRP fraction determined by SDS-PAGE indicates a good selectivity of VPC-MNPs for CRP binding. These results demonstrate the feasibility of using ligand-functionalized MNPs for rapid, easy, and efficient protein isolation. © 2013 Copyright Taylor and Francis Group, LLC. -
dc.publisher Taylor and Francis Ltd. -
dc.title Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum -
dc.type Article -
dc.identifier.doi 10.1080/01496395.2013.807516 -
dc.identifier.scopusid 2-s2.0-84886426070 -
dc.identifier.bibliographicCitation Separation Science and Technology, v.48, no.17, pp.2600 - 2607 -
dc.subject.keywordAuthor affinity isolation -
dc.subject.keywordAuthor C-reactive protein (crp) -
dc.subject.keywordAuthor magnetic nanoparticle -
dc.subject.keywordAuthor phosphocholine -
dc.subject.keywordAuthor protein purification -
dc.subject.keywordPlus IRON-OXIDE NANOPARTICLES -
dc.subject.keywordPlus AFFINITY-CHROMATOGRAPHY -
dc.subject.keywordPlus SEPARATION -
dc.subject.keywordPlus PURIFICATION -
dc.subject.keywordPlus BEADS -
dc.subject.keywordPlus ACID -
dc.subject.keywordPlus PARTICLES -
dc.subject.keywordPlus LIGANDS -
dc.citation.endPage 2607 -
dc.citation.number 17 -
dc.citation.startPage 2600 -
dc.citation.title Separation Science and Technology -
dc.citation.volume 48 -
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Division of Biomedical Technology 1. Journal Articles
Division of AI, Big data and Block chain 1. Journal Articles

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