Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nam, In Ho | ko |
dc.contributor.author | Nam, Hong Gil | ko |
dc.contributor.author | Zare, Richard N | ko |
dc.date.accessioned | 2018-02-05T04:11:30Z | - |
dc.date.available | 2018-02-05T04:11:30Z | - |
dc.date.created | 2018-01-19 | - |
dc.date.issued | 2018-01 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences of the United States of America, v.115, no.1, pp.36 - 40 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/5600 | - |
dc.description.abstract | Aqueous microdroplets (< 1.3 mu m in diameter on average) containing 15 mM D-ribose, 15 mM phosphoric acid, and 5 mM of a nucleobase (uracil, adenine, cytosine, or hypoxanthine) are electrosprayed from a capillary at + 5 kV into a mass spectrometer at room temperature and 1 atm pressure with 3 mM divalent magnesium ion (Mg2+) as a catalyst. Mass spectra show the formation of ribonucleosides that comprise a four-letter alphabet of RNA with a yield of 2.5% of uridine (U), 2.5% of adenosine (A), 0.7% of cytidine (C), and 1.7% of inosine (I) during the flight time of similar to 50 mu s. In the case of uridine, no catalyst is required. An aqueous solution containing guanine cannot be generated under the same conditions given the extreme insolubility of guanine in water. However, inosine can base pair with cytidine and thus substitute for guanosine. Thus, a full set of ribonucleosides to generate the purine-pyrimidine base pairs A-U and I-C are spontaneously generated in aqueous microdroplets under similar mild conditions. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | D-RIBOSE 1-PHOSPHATE | - |
dc.subject | CHARGED MICRODROPLETS | - |
dc.subject | ATMOSPHERIC AEROSOLS | - |
dc.subject | MASS-SPECTROMETRY | - |
dc.subject | ORIGIN | - |
dc.subject | LIFE | - |
dc.subject | SALVAGE | - |
dc.subject | ELECTROSPRAY | - |
dc.subject | INTERFACE | - |
dc.subject | CHEMISTRY | - |
dc.title | Abiotic synthesis of purine and pyrimidine ribonucleosides in aqueous microdroplets | - |
dc.type | Article | - |
dc.identifier.doi | 10.1073/pnas.1718559115 | - |
dc.identifier.wosid | 000419128700023 | - |
dc.identifier.scopusid | 2-s2.0-85040201128 | - |
dc.type.local | Article(Overseas) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.contributor.nonIdAuthor | Nam, In Ho | - |
dc.contributor.nonIdAuthor | Zare, Richard N | - |
dc.identifier.citationVolume | 115 | - |
dc.identifier.citationNumber | 1 | - |
dc.identifier.citationStartPage | 36 | - |
dc.identifier.citationEndPage | 40 | - |
dc.identifier.citationTitle | Proceedings of the National Academy of Sciences of the United States of America | - |
dc.type.journalArticle | Article | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Nam, Hong Gil | - |
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