Cited 1 time in webofscience Cited 1 time in scopus

Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution

Title
Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution
Authors
Kim, Jae YoungSeo, Eun SeokKim, Hyun MinPark, Ji WonLim, Dong KwonMoon, Dae Won
DGIST Authors
Kim, Jae Young; Kim, Hyun MinMoon, Dae Won
Issue Date
2017-12
Citation
Nature Communications, 8(1)
Type
Article
Article Type
Article
Keywords
adeninebeta cyclodextrincholesterolglycerolipidgold nanorodmonoacylglycerolphosphorylcholinesphinganinesphingolipidsphingosineatmospheric pressurecells and cell componentsimaging methodionizationmass spectrometrymolecular analysisnanoparticlespatial resolutionadultairflowanimal tissueArticleatmospheric pressureconfocal laser scanning microscopycontrolled studydesorptionendocytosisenvironmental temperaturefluorescence imaginghippocampal tissueionizationmass spectrometrymousenonhumansurface propertytissue slicetransmission electron microscopy
ISSN
2041-1723
Abstract
We report a high spatial resolution mass spectrometry (MS) system that allows us to image live hippocampal tissue slices under open-air atmospheric pressure (AP) and ambient temperature conditions at the subcellular level. The method is based on an efficient desorption process by femtosecond (fs) laser assisted with nanoparticles and a subsequent ionization step by applying nonthermal plasma, termed AP nanoparticle and plasma assisted laser desorption ionization (AP-nanoPALDI) MS method. Combining the AP-nanoPALDI with microscopic sample scanning, MS imaging with spatial resolution of 2.9 μm was obtained. The observed AP-nanoPALDI MS imaging clearly revealed the differences of molecular composition between the apical and basal dendrite regions of a hippocampal tissue. In addition, the AP-nanoPALDI MS imaging showed the decrease of cholesterol in hippocampus by treating with methyl β-cyclodextrin, which exemplifies the potential of AP-nanoPALDI for live tissue imaging for various biomedical applications without any chemical pretreatment and/or labeling process. © 2017 The Author(s).
URI
http://hdl.handle.net/20.500.11750/5619
DOI
10.1038/s41467-017-02216-6
Publisher
Nature Publishing Group
Related Researcher
Files:
There are no files associated with this item.
Collection:
Department of New BiologyNanoBio Imaging Laboratory1. Journal Articles
Companion Diagnostics and Medical Technology Research Group1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE