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From Homogeneity to Turing Pattern: Kinetically Controlled Self-Organization of Transmembrane Protein

Title
From Homogeneity to Turing Pattern: Kinetically Controlled Self-Organization of Transmembrane Protein
Author(s)
Lee, Wonhee JohnKim, Soo JinAhn, YongdeokPark, JiseongJin, SiwooJang, JuheeJeong, JinjuPark, MinsooLee, Young-SamLee, JunyeopSeo, Daeha
Issued Date
2024-01
Citation
Nano Letters, v.24, no.6, pp.1882 - 1890
Type
Article
Author Keywords
PLVAPreaction−diffusionsingle-molecule trackingself-organizationpattern formation
Keywords
CELLDIAPHRAGMSDIMERIZATIONACTIVATIONTRACKINGMOTIONSTATESPV-1REACTION-DIFFUSION MODELPHASE-SEPARATION
ISSN
1530-6984
Abstract
Understanding the spatial organization of membrane proteins is crucial for unraveling key principles in cell biology. The reaction-diffusion model is commonly used to understand biochemical patterning; however, applying reaction-diffusion models to subcellular phenomena is challenging because of the difficulty in measuring protein diffusivity and interaction kinetics in the living cell. In this work, we investigated the self-organization of the plasmalemma vesicle-associated protein (PLVAP), which creates regular arrangements of fenestrated ultrastructures, using single-molecule tracking. We demonstrated that the spatial organization of the ultrastructures is associated with a decrease in the association rate by actin destabilization. We also constructed a reaction-diffusion model that accurately generates a hexagonal array with the same 130 nm spacing as the actual scale and informs the stoichiometry of the ultrastructure, which can be discerned only through electron microscopy. Through this study, we integrated single-molecule experiments and reaction-diffusion modeling to surpass the limitations of static imaging tools and proposed emergent properties of the PLVAP ultrastructure. © 2024 American Chemical Society
URI
http://hdl.handle.net/20.500.11750/56539
DOI
10.1021/acs.nanolett.3c03637
Publisher
American Chemical Society
Related Researcher
  • 이영삼 Lee, Young-Sam
  • Research Interests DNA replication and repair; Restoration of cellular senescence; Structural and functional relationship of proteins
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Appears in Collections:
Department of Physics and Chemistry SMALL LAB(Single Molecule Approaches to ceLL Lab) 1. Journal Articles
Department of New Biology Senescence-Associated Mechanism Lab 1. Journal Articles

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