Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kang, Moo Seok | - |
dc.contributor.author | Kim, Sang Yeol | - |
dc.contributor.author | Kim, Hyo Jung | - |
dc.contributor.author | Shrestha, Pravesh | - |
dc.contributor.author | Yun, Ji-Hye | - |
dc.contributor.author | Phee, Bong-Kwan | - |
dc.contributor.author | Lee, Weontae | - |
dc.contributor.author | Nam, Hong Gil | - |
dc.contributor.author | Chang, Ik Soo | - |
dc.date.available | 2018-02-26T08:44:11Z | - |
dc.date.created | 2018-02-22 | - |
dc.date.issued | 2018-01 | - |
dc.identifier.citation | Cell Reports, v.22, no.5, pp.1141 - 1150 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/5883 | - |
dc.description.abstract | The affinity of transcription factors (TFs) for their target DNA is a critical determinant of gene expression. Whether the DNA-binding domain (DBD) of TFs alone can regulate binding affinity to DNA is an important question for identifying the design principle of TFs. We studied ANAC019, a member of the NAC TF family of proteins in Arabidopsis, and found a well-conserved histidine switch located in its DBD, which regulates both homodimerization and transcriptional control of the TF through H135 protonation. We found that the removal of a C-terminal intrinsically disordered region (IDR) in the TF abolished the pH-dependent binding of the N-terminal DBD to DNA. We propose a mechanism in which long-range electrostatic interactions between DNA and the negatively charged C-terminal IDR turns on the pH dependency of the DNA-binding affinity of the N-terminal DBD. Kang et al. find a histidine switch in the DNA-binding N-domain of the transcription factor ANAC019 that regulates both pH-dependent homodimerization and DNA binding. They propose that long-range electrostatic interactions between DNA and the negatively charged C-terminal turns on the pH dependency of the DNA-binding affinity of the N-terminal DNA-binding domain. © 2018 The Authors | - |
dc.language | English | - |
dc.publisher | Cell Press | - |
dc.title | The C-Domain of the NAC Transcription Factor ANAC019 Is Necessary for pH-Tuned DNA Binding through a Histidine Switch in the N-Domain | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.celrep.2018.01.002 | - |
dc.identifier.wosid | 000423898500005 | - |
dc.identifier.scopusid | 2-s2.0-85044864664 | - |
dc.type.local | Article(Overseas) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.citation.publicationname | Cell Reports | - |
dc.contributor.nonIdAuthor | Kang, Moo Seok | - |
dc.contributor.nonIdAuthor | Kim, Sang Yeol | - |
dc.contributor.nonIdAuthor | Kim, Hyo Jung | - |
dc.contributor.nonIdAuthor | Shrestha, Pravesh | - |
dc.contributor.nonIdAuthor | Yun, Ji-Hye | - |
dc.contributor.nonIdAuthor | Phee, Bong-Kwan | - |
dc.contributor.nonIdAuthor | Lee, Weontae | - |
dc.identifier.citationVolume | 22 | - |
dc.identifier.citationNumber | 5 | - |
dc.identifier.citationStartPage | 1141 | - |
dc.identifier.citationEndPage | 1150 | - |
dc.identifier.citationTitle | Cell Reports | - |
dc.type.journalArticle | Article | - |
dc.description.isOpenAccess | Y | - |
dc.subject.keywordPlus | ARABIDOPSIS-THALIANA | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | FACTOR FAMILY | - |
dc.subject.keywordPlus | MEMBER | - |
dc.contributor.affiliatedAuthor | Kang, Moo Seok | - |
dc.contributor.affiliatedAuthor | Kim, Sang Yeol | - |
dc.contributor.affiliatedAuthor | Kim, Hyo Jung | - |
dc.contributor.affiliatedAuthor | Shrestha, Pravesh | - |
dc.contributor.affiliatedAuthor | Yun, Ji-Hye | - |
dc.contributor.affiliatedAuthor | Phee, Bong-Kwan | - |
dc.contributor.affiliatedAuthor | Lee, Weontae | - |
dc.contributor.affiliatedAuthor | Nam, Hong Gil | - |
dc.contributor.affiliatedAuthor | Chang, Ik Soo | - |