Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Kim, Kyu Hyung | - |
| dc.contributor.author | Hong, Myeongjin | - |
| dc.date.accessioned | 2018-03-14T02:03:24Z | - |
| dc.date.available | 2018-03-14T02:03:24Z | - |
| dc.date.issued | 2018 | - |
| dc.identifier.uri | http://dgist.dcollection.net/common/orgView/200000005426 | en_US |
| dc.identifier.uri | http://hdl.handle.net/20.500.11750/6024 | - |
| dc.description.abstract | Experiences during early development can influence neuronal functions and modu-late adult behaviors. However, the molecular mechanisms underlying the long-term be-havioral effects of these early experiences are not fully understood. The C. elegans ascr#3 pheromone triggers avoidance behavior in adult hermaphrodites. Here, I show that hermaphrodites that are briefly exposed to ascr#3 immediately af-ter birth exhibit increased ascr#3-specific avoidance as adults indicating that ascr#3-experienced animals form a long lasting memory or imprint of this early ascr#3 exposure. ascr#3 imprinting is mediated by increased synaptic activity between the ascr#3-sensing ADL neurons and their post-synaptic SMB motor neuron partners via increased expression of the odr-2 GPI-linked signaling and avr-14 glutamate-gated chloride channel genes in the SMB neurons. My study suggests that the memory for early ascr#3 experience is imprinted via al-teration of a single synaptic connection, that in turn shapes experience-dependent plastici-ty in adult ascr#3 responses. ⓒ 2017 DGIST |
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| dc.description.statementofresponsibility | open | - |
| dc.description.tableofcontents | Ⅰ. Introduction 1-- 1.1 Neuroconnectome 1-- 1.2 Synaptic plasticity 1-- 1.3 Studying of C. elegans as a model system 2-- 1.4 C. elegans pheromones 3-- 1.5 Early pheromone-experienced study of C. elegans 4-- II. Experimental Method & Materials 5-- 2.1 Experimental Model and Subject Details 5-- 2.2 Method Details 5-- 2.2.1 Generation of C. elegans transgenic lines 5-- 2.2.2 Pheromone imprinting 6-- 2.2.3 Post-dauer assay 7-- 2.2.4 Behavioral assay 7-- 2.2.5 In vivo calcium imaging 8-- 2.2.6 Levamizole treatment 8-- 2.2.7 Heat shock treatment 9-- 2.3 Quantification and Statistical Analysis 9-- 2.3.1 Representative images 9-- 2.3.2 GFP quantification 9-- 2.3.3 Statistical tests 10-- III. Result 11-- 3.1 Adult C. elegans transiently exposed to ascr#3 during larval stages ex-hibits increased acsr#3 pheromone avoidance 11-- 3.1.1 Pheromone imprinting assay of naive and pre-exposed worms 11-- 3.1.2 Optimal ascr#3 concentration of pre-exposure effect 13-- 3.1.3 Early ascr#3 experience of males 14-- 3.2 The memory for ascr#3 is acquired during the L1 larval stage 14-- 3.2.1 ascr#3 imprinting is L1 specific 14-- 3.2.2 The critical time for imprinting of L1 stage 15-- 3.2.3 ascr#3 imprinting is pheromone specific 16-- 4.1 odr-2 Acts in the SMB neurons to increase ascr#3 avoidance in ascr#3-imprinted animals 20-- 4.1.1 Candidate gene search 20-- 4.1.2 odr-2 recue study 22-- 4.1.3 SMB sensory/inter/motor neurons 24-- 4.1.4 Association of SMB and Imprining 25-- 4.2 ascr#3-induced responses in the ADL chemosensory neurons are unal-tered in ascr#3 imprinted worms 25-- 4.2.1 ADL Ca²⁺ Imaging 25-- 4.2.2 ADL Ca²⁺ Imaging in odr-2 27-- 4.2.3 AIB, AVD, AVA Ca²⁺ imaging 27-- 4.3 SMB mediates increased ascr#3 avoidance in ascr#3 imprinted ani-mals 30-- 4.3.1 SMB Ca²⁺ Imaging 30-- 4.3.2 Levamisole-treated SMB Ca²⁺ Imaging 31-- 4.3.3 ADLp::TeTx-treated SMB Ca²⁺ Imaging 32-- 4.3.4 SMB Ca²⁺ Imaging in odr-2 34-- 4.4 Upregulation of odr-2 expression in SMB of ascr#3-imprinted L1 lar-vae is sufficient for ascr#3 imprinting 34-- 4.4.1 GFP quantification of naive and imprinted C. elegans 34-- 4.4.2 Heatshock study of non-imprinted animals 35-- 4.4.3 avr-14 study 36-- IV. Discussion 38-- V. Conclusion 40 |
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| dc.format.extent | 48 | - |
| dc.language | eng | - |
| dc.publisher | DGIST | - |
| dc.subject | Neuroconnectome | - |
| dc.subject | Synaptic plasticity | - |
| dc.subject | Imprinting | - |
| dc.subject | Molecular genetics | - |
| dc.subject | 뉴로커넥톰 | - |
| dc.subject | 각인 | - |
| dc.subject | 시냅스 | - |
| dc.title | Early sensory-experience modulates pheromone-mediated behaviors of adult C. elegans | - |
| dc.title.alternative | 페로몬 감각 각인의 기초가 되는 회로 메커니즘연구 | - |
| dc.type | Thesis | - |
| dc.identifier.doi | 10.22677/thesis.200000005426 | - |
| dc.description.alternativeAbstract | 본 논문은 전체 게놈이 최초로 알려졌으며 유일무이한 개체수준의 뉴로커넥톰이 구축된 예쁜꼬마선충을 모델시스템으로 연구를 진행하였다. 이전 연구들은 각 신경들의 구조와 기능에 대한 미시적 수준에 머물러, 뉴로커넥톰의 역할과 기능에 관한 거시적 수준에서의 연구는 미약한 실정이었다. 본 논문은 세계최초로 페로몬에 대한 ‘각인’이라는 장기기억 현상을 발견하였다. 먼저 이러한 행동가소성을 야기하는 원인 유전자를 찾아 신경세포 수준에서의 접근법을 모색 하였고, 그 후 분자생물학 및 유전학적 방법을 기반으로 관련 신경회로의 작동원리를 규명하여 스마트 뉴로커넥톰을 구축하는데 일조하였다. 따라서 본 논문은 뉴로커넥톰의 작동원리 규명, 향후 뇌신경계 기능 구현, 다양한 신경정신 질환의 원인 규명 및 치료로의 활용에 획기적 단서를 제공할 것이다. | - |
| dc.description.degree | Doctor | - |
| dc.contributor.department | Brain and Cognitive Sciences | - |
| dc.contributor.coadvisor | Kea Joo Lee | - |
| dc.date.awarded | 2018. 2 | - |
| dc.publisher.location | Daegu | - |
| dc.description.database | dCollection | - |
| dc.date.accepted | 2018-01-05 | - |
| dc.contributor.alternativeDepartment | 대학원 뇌인지과학전공 | - |
| dc.contributor.alternativeName | 홍명진 | - |
| dc.contributor.alternativeName | 김규형 | - |
| dc.contributor.alternativeName | 이계주 | - |
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