Title

Atg7-deficiency in hypothalamic tanycytes disrupts energy homeostasis

Alternative Title

시상하부 띠뇌실막세포의 Atg7 결핍으로 인한 에너지 항상성 조절에 관한 연구

DGIST Authors

Woong Jeong ; Eun-Kyung Kim ; Byung-Hoon Lee

Advisor

김은경

Co-Advisor(s)

Byung-Hoon Lee

Issued Date

2026

Awarded Date

2026-08-01

Type

Thesis

Description

Tanycytes, autophagy, Atg7, Rax-creERT2, feeding behavior, body weight regulation

Table Of Contents

List of Contents

Abstract ⅰ
List of contents iii
List of tables vi
List of figures vii
List of abbreviations viii


Ⅰ. Introduction 1
1. Role of hypothalamic neurons in energy expenditure and food intake 6
2. Structural and functional characterization of hypothalamic tanycytes 7
3. Overview of autophagy process 8
4. Aims of the study 10

Ⅱ. Materials and Methods 11
1. Animal studies 11
2. Intraperitoneal injections of Tamoxifen 11
3. Genotyping 11
4. Immunohistochemistry and Fluorescence microscopy 12
5. Measurements of food intake and body weight 13
6. Statistical analysis 13

Ⅲ. Results 15
1. Generation and validation of inducible Rax-CreERT2; Atg7fl/fl mice 15
2. Validation of tanycytes-specific activity of Rax-CreERT2 17
3. Validation of tanycytes-specific Atg7 ablation 19
4. Tanycytes-specific Atg7 deletion suppresses autophagy in hypothalamic tanycytes 22
5. Tanycytes-specific deletion of Atg7 increases body weight gain and food intake under
normal chow conditions 25

Ⅳ. Discussion 28

Ⅴ. Conclusion 33

References 34

Abstract in Korean 38

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/60767
http://dgist.dcollection.net/common/orgView/200001015038

DOI

10.22677/THESIS.200001015038

Degree

Master

Department

Department of Brain Sciences

Publisher

DGIST

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