Title

SGK3–ULK1 pathway induces p62 body formation following corticosterone treatment in adult hippocampal neural stem cells

Alternative Title

코르티코스테론 처리 후 성체 해마 신경줄기세포에서 SGK3- ULK1 경로는 p62 body 형성을 유도

DGIST Authors

Juhee Choi ; Seong-Woon Yu ; Byung-Hoon Lee

Advisor

유성운

Co-Advisor(s)

Byung-Hoon Lee

Issued Date

2026

Awarded Date

2026-08-01

Type

Thesis

Description

Adult hippocampal neural stem cells, Autophagy, Corticosterone, Liquid-liquid phase separation, p62/SQSTM1 body, serum/glucocorticoid kinase 3, Unc-51-like autophagy activating kinase 1

Table Of Contents

List of Contents
Abstract i
List of contents ii
List of figures iii

Ⅰ. Introduction
II. Materials and Methods
2.1 Antibodies and reagents 8
2.2 Constructs 8
2.3 Cell Culture 8
2.4 Generation of stable knockdown or knockout ahNSCs 8
2.5 Western blotting 9
2.6 Confocal imaging and analysis 9
2.7 Live imaging 9
2.8 Co-immunoprecipitation assay 9
2.9 Proximity ligation assay （PLA） 10
2.10 Cell Death Assay 10
2.11 Statistics 10

III. Results
3.1 CORT induces formation of p62 bodies. 11
3.2 SGK3 is required for CORT-induced p62 body formation. 13
3.3 SGK3 does not directly interact with p62 but is in close spatial proximity. 16
3.4 ULK1 contributes to CORT-induced p62 body formation. 18
3.5 SGK3 functions upstream of ULK1 for p62 body formation. 21

IV. Discussion 26
V. References 28
Abstract in Korean 31

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/60768
http://dgist.dcollection.net/common/orgView/200001017895

DOI

10.22677/THESIS.200001017895

Degree

Master

Department

Department of Brain Sciences

Publisher

DGIST

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