Title

Real-time computation to unmix signal crosstalk of multiple fluorescence indicators

Alternative Title

다중 형광 지표들의 신호 중첩을 분리하기 위한 실시간 계산 연구

DGIST Authors

Min Yong Lee ; Kwang Lee ; Yong-Seok Oh

Advisor

이광

Co-Advisor(s)

Yong-Seok Oh

Issued Date

2026

Awarded Date

2026-08-01

Type

Thesis

Description

spectrometer-based photometry, dual-color recording, spectral unmixing, real-time regression algorithm

Table Of Contents

Ⅰ. Introduction 1
ⅠI. Methods 2
2.1 Animal 2
2.2 Surgical procedures 2
2.3 Behavioral task 3
2.4 Fiber photometry recording 3
2.5 Signal processing and data analysis 4
2.6 Immunohistochemistry 5
2.7 Quantification and Statistical Analysis 6
ⅠII. Results 6
3.1 Development of a Spectrometer-Based Fiber Photometry System 6
3.2 Emission Spectral Distortion in Mixed Fluorophore Conditions 9
3.3 Real-Time Correction of Spectral Crosstalk in Dual-Color Photometry System 13
3.4 Application to Multiple Target Sensors 18
3.5 Sensor Compatibility of the Correction Algorithm 20
ⅠV. Discussion 23
V. Reference 25
Abstract in Korean 29

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/60771
http://dgist.dcollection.net/common/orgView/200001014860

DOI

10.22677/THESIS.200001014860

Degree

Master

Department

Department of Brain Sciences

Publisher

DGIST

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