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Proteasome subunit RPT2a promotes PTGS through repressing RNA quality control in Arabidopsis

Title
Proteasome subunit RPT2a promotes PTGS through repressing RNA quality control in Arabidopsis
Authors
Kim, Myung-HeeJeon, Ji. eun.Lee, SeulbeeLee, Jae. Ho.Gao, LeiLee, Byung-HoonPark, Jeong MeeKim, Yun JuKwak, June Myoung
DGIST Authors
Lee, Byung-HoonKwak, June Myoung
Issue Date
2019-12
Citation
Nature Plants, 5(12), 1273-1282
Type
Article
Article Type
Article
Keywords
CUTICULAR WAX BIOSYNTHESISSMALL INTERFERING RNASDNA METHYLATIONGENETURNOVEREXOSOMEPLANTSDEGRADATIONGENERATIONARGONAUTE1
ISSN
2055-026X
Abstract
RNA quality control (RQC) and post-transcriptional gene silencing (PTGS) target and degrade aberrant endogenous RNAs and foreign RNAs, contributing to homeostasis of cellular RNAs. In plants, RQC and PTGS compete for foreign and selected endogenous RNAs; however, little is known about the mechanism interconnecting the two pathways. Using a reporter system designed for monitoring PTGS, we revealed that the 26S proteasome subunit RPT2a enhances transgene PTGS by promoting the accumulation of transgene-derived short interfering RNAs without affecting their biogenesis. RPT2a physically associated with a subset of RQC components and downregulated the protein level. Overexpression of the RQC components interfered with transgene silencing, and impairment of the RQC machinery reinforced transgene PTGS attenuated by rpt2a. Overall, we demonstrate that the 26S proteasome subunit RPT2a promotes PTGS by repressing the RQC machinery to control foreign RNAs. © 2019, The Author(s), under exclusive licence to Springer Nature Limited.
URI
http://hdl.handle.net/20.500.11750/10932
DOI
10.1038/s41477-019-0546-1
Publisher
Palgrave Macmillan Ltd.
Related Researcher
  • Author Lee, Byung-Hoon Lab of Protein Homeostasis and Drug Discovery
  • Research Interests Ubiquitin-proteasome system; Protein homeostasis; Small-molecule chemical screening and drug discovery in human disease
Files:
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Collection:
Department of New BiologyLab of Protein Homeostasis and Drug Discovery1. Journal Articles
Department of New BiologyKWAK_LAB(Lab of Cell Signailng & Development)1. Journal Articles


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