Cited 5 time in
Cited 5 time in
Proteasome subunit RPT2a promotes PTGS through repressing RNA quality control in Arabidopsis
- Proteasome subunit RPT2a promotes PTGS through repressing RNA quality control in Arabidopsis
- Kim, Myung-Hee; Jeon, Ji. eun.; Lee, Seulbee; Lee, Jae. Ho.; Gao, Lei; Lee, Byung-Hoon; Park, Jeong Mee; Kim, Yun Ju; Kwak, June Myoung
- DGIST Authors
- Lee, Byung-Hoon; Kwak, June Myoung
- Issue Date
- Nature Plants, 5(12), 1273-1282
- Article Type
- CUTICULAR WAX BIOSYNTHESIS; SMALL INTERFERING RNAS; DNA METHYLATION; GENE; TURNOVER; EXOSOME; PLANTS; DEGRADATION; GENERATION; ARGONAUTE1
- RNA quality control (RQC) and post-transcriptional gene silencing (PTGS) target and degrade aberrant endogenous RNAs and foreign RNAs, contributing to homeostasis of cellular RNAs. In plants, RQC and PTGS compete for foreign and selected endogenous RNAs; however, little is known about the mechanism interconnecting the two pathways. Using a reporter system designed for monitoring PTGS, we revealed that the 26S proteasome subunit RPT2a enhances transgene PTGS by promoting the accumulation of transgene-derived short interfering RNAs without affecting their biogenesis. RPT2a physically associated with a subset of RQC components and downregulated the protein level. Overexpression of the RQC components interfered with transgene silencing, and impairment of the RQC machinery reinforced transgene PTGS attenuated by rpt2a. Overall, we demonstrate that the 26S proteasome subunit RPT2a promotes PTGS by repressing the RQC machinery to control foreign RNAs. © 2019, The Author(s), under exclusive licence to Springer Nature Limited.
- Palgrave Macmillan Ltd.
- Related Researcher
Lab of Protein Homeostasis and Drug Discovery
Ubiquitin-proteasome system; Protein homeostasis; Small-molecule chemical screening and drug discovery in human disease
There are no files associated with this item.
- Department of New BiologyLab of Protein Homeostasis and Drug Discovery1. Journal Articles
Department of New BiologyLab of Cell Signaling and Development1. Journal Articles
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.