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Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons

Title
Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons
Authors
Um, Ji WonHan, Kyung AhChoi, Se-YoungKo, Jaewon
DGIST Authors
Um, Ji Won; Han, Kyung Ah; Choi, Se-Young; Ko, Jaewon
Issue Date
2020-09
Citation
STAR Protocols, 1(2), 100095
Type
Article
Article Type
Protocol
ISSN
2666-1667
Abstract
Clustering of synaptic vesicles along the neuronal axons is a critical mechanism underpinning proper synaptic transmission. Here, we provide a detailed protocol for analyzing the distribution of synaptic vesicles in presynaptic boutons of cultured neurons. The protocol covers preparation of cultured neurons, expression of synaptic vesicle-enriched proteins, and quantification procedures. Utilizing neurons from postnatal transgenic mice, this method can be applied to investigate the roles of synaptic genes in regulating vesicle dynamics at synaptic sites. For complete details on the use and execution of this protocol, please refer to Han et al. (2020a). © 2020 The Author(s)
URI
http://hdl.handle.net/20.500.11750/12578
DOI
10.1016/j.xpro.2020.100095
Publisher
Elsevier
Related Researcher
  • Author Ko, Jaewon Laboratory of Synapse Formation and Function
  • Research Interests Synapse Formation and Function; Neural Circuits; 뇌질환; animal model
Files:
Collection:
Department of Brain and Cognitive SciencesSynapse Disorder Laboratory1. Journal Articles
Department of Brain and Cognitive SciencesLaboratory of Synapse Formation and Function1. Journal Articles


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