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Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells

Title
Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells
Author(s)
Kim, Jung-HeeKim, Hye-RimLee, Bo-RamChoi, Eun-SookIn, Su-IlKim, Eunjoo
Issued Date
2015-08
Citation
International Journal of Nanomedicine, v.10, no.1, pp.5513 - 5528
Type
Article
Author Keywords
comet assayDNA damageexosomehigh-throughput screeninglead sulfide quantum dots (PbS QDs)nanoparticle toxicity
Keywords
COMET ASSAYDNA-DAMAGECANCER-CELLSMULTIVESICULAR BODIESEXPRESSION PROFILESMICRORNA EXPRESSIONTUMOR-CELLSPROTEINSMIRNAVESICLES
ISSN
1176-9114
Abstract
Lead sulfide (PbS) quantum dots (QDs) have been applied in the biomedical area because they offer an excellent platform for theragnostic applications. In order to comprehensively evaluate the biocompatibility of PbS QDs in human cells, we analyzed the exosomes secreted from cells because exosomes are released during cellular stress to convey signals to other cells and serve as a reservoir of enriched biomarkers. PbS QDs were synthesized and coated with 3-mercaptopropionic acid (MPA) to allow the particles to disperse in water. Exosomes were isolated from HEK293 cells treated with PbS–MPA at concentrations of 0 µg/mL, 5 µg/mL, and 50 µg/mL, and the exosomal expression levels of miRNAs and proteins were analyzed. As a result, five miRNAs and two proteins were proposed as specific exosomal biomarkers for the exposure of HEK293 cells to PbS–MPA. Based on the pathway analysis, the molecular signature of the exosomes suggested that PbS–MPA QDs had carcinogenic activity. The comet assay and expression of molecular markers, such as p53, interleukin (IL)-8, and C-X-C motif chemokine 5, indicated that DNA damage occurred in HEK293 cells following PbS–MPA exposure, which supported the carcinogenic activity of the particles. In addition, there was obvious intensification of miRNA expression signals in the exosomes compared with that of the parent cells, which suggested that exosomal biomarkers could be detected more sensitively than those of whole cellular extracts. © 2015 Kim et al.
URI
http://hdl.handle.net/20.500.11750/1581
DOI
10.2147/IJN.S89593
Publisher
Dove Medical Press Ltd
Related Researcher
  • 인수일 In, Su-Il
  • Research Interests CO2 conversion to hydrocarbon fuels; Water splitting for hydrogen generation; Quantum dot devices; Dye sensitized solar cells; Environmental remediation; Synthesis of functional nanomaterials; CO2 연료전환; 수소생산을 위한 광전기화학적 물분해; 양자점 태양전지; 염료감응 태양전지; 공해물질 저감연구; 기능성 나노소재 개발
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Appears in Collections:
Department of Energy Science and Engineering Green and Renewable Energy for Endless Nature(GREEN) Lab 1. Journal Articles
Companion Diagnostics and Medical Technology Research Group 1. Journal Articles
Division of AI, Big data and Block chain 1. Journal Articles

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