Title

Three-dimensional microelectrodes for subretinal stimulation

Alternative Title

망막하 자극을 위한 3차원 형태의 미세전극들

DGIST Authors

Hee Won Seo ; Sohee Kim ; Han Kyoung Choe

Advisor

김소희

Co-Advisor(s)

Han Kyoung Choe

Issued Date

2022

Awarded Date

2022/08

Citation

Hee Won Seo. (2022). Three-dimensional microelectrodes for subretinal stimulation. doi: 10.22677/thesis.200000627656

Type

Thesis

Subject

retinal prosthesis, subretinal implant, neural electrodes, 3D microelectrodes, transparent base

Description

retinal prosthesis, subretinal implant, neural electrodes, 3D microelectrodes, transparent base

Abstract

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Table Of Contents

Chapter Ⅰ. Introduction 1
1. Background 2
1.1 Retinal disease 2
1.2 Visual prosthesis 4
2. Objective of research 13
3. References 15
Chapter Ⅱ. 3D microelectrodes with square arrangement 18
1. Introduction 19
2. Methods 23
2.1 Modeling and simulation to optimize the electrode geometry 23
2.2 Design and fabrication of the transparent 3D microelectrode array 28
2.2.1 Design of the microelectrodes 28
2.2.2 Fabrication of the microelectrodes 29
2.3 Electrochemical characterization 35
2.4 In-vitro experiment 36
2.4.1 Retina preparation 36
2.4.2 Multi-electrode recording system 39
2.4.3 Electrical stimulation 39
2.4.4 Data analysis 40
3. Results and discussion 42
3.1 Simulated results of the electrode designs 42
3.2 Fabrication results of the electrodes 45
3.3 Electrode impedance and cyclic voltammetry 47
3.4 In-vitro subretinal stimulation 49
4. Conclusion 54
5. References 55
Chapter Ⅲ. 3D microelectrodes with hexagonal arrangement 60
1. Introduction 61
2. Materials and methods 64
2.1 Design of subretinal electrode array 64
2.2 Generation of silicon pillars and transparent base 65
2.3 Generation of connection pads and parylene insulation 68
2.4 Electrochemical characterization 71
2.5 Material properties 72
2.6 Improved electrodes and accelerated aging test 73
2.7 In-vitro experiment 75
3. Results and discussion 78
3.1 Fabrication results of the electrodes 78
3.2 Electrode impedance and cyclic voltammetry 84
3.3 Properties of the fabricated electrodes 87
3.3.1 Transmittance and reflectance of materials 87
3.3.2 Long-term EIS results and CSC 90
3.4 In-vitro subretinal stimulation 92
3.4.1 Single stimulation 92
3.4.2 Stimulation with the opposite current 94
4. Conclusion 105
5. References 107
Chapter Ⅳ. Biocompatibility evaluation of 3D subretinal electrodes 111
1. Introduction 112
2. Materials and methods 115
2.1 Subretinal implants 115
2.2 Animals and surgical procedure 118
2.3 Fundus infrared reflectance and SD-OCT 120
2.4 Immunohistochemical examination (tissue clearing method) 124
3. Results 126
3.1 SD-OCT imaging and TRL thicknesses 126
3.2 Immunohistochemical analysis 135
4. Discussion 138
5. Conclusion 147
6. References 148
Chapter Ⅴ. Conclusion and future direction 152
1. Conclusion 153
2. Future direction 158
3. References 160
Appendix 162
요약문 169

URI

http://dgist.dcollection.net/common/orgView/200000627656
http://hdl.handle.net/20.500.11750/16775

DOI

10.22677/thesis.200000627656

Degree

Doctor

Department

Department of Robotics and Mechatronics Engineering

Publisher

DGIST

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