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Fluid-driven DNA stretching for single-molecule studies on chromatin-associated proteins

Title
Fluid-driven DNA stretching for single-molecule studies on chromatin-associated proteins
Author(s)
Heo, WonjeSeo, JeonghwanLee, YoonheeKim, Yoori
Issued Date
2022-12
Citation
Biochemical and Biophysical Research Communications, v.634, pp.122 - 128
Type
Article
Author Keywords
Single-molecule imagingDNA stretchingLithographyDNA-Protein interaction
Keywords
CURTAINSREPLICATIONMICROSCOPYSEPARATIONARRAYS
ISSN
0006-291X
Abstract
There have been many attempts to understand the central principle of life mediated by DNA-protein interactions surrounding complex environments. Still, the mechanistic insight of individual protein functions has been lacking in traditional ensemble assays. Thus, techniques visualizing a single molecule have emerged to uncover the discrete roles of DNA-protein interactions and their biophysical properties. This paper will review the advances in single-molecule tools imaging long genomic DNA and their applications in studying dynamic protein interactions. We focus on the three representative techniques, including molecular combing, nanochannel confinement, and DNA curtain assays, which use fluid-driven force to elongate the individual DNA. We provide an integrated perspective and a direction for future use to those who want to observe single DNA molecules along with their cellular factor of interest and employ them for dissecting protein function. © 2022 Elsevier Inc.
URI
http://hdl.handle.net/20.500.11750/17230
DOI
10.1016/j.bbrc.2022.10.023
Publisher
Academic Press
Related Researcher
  • 이윤희 Lee, Yoonhee
  • Research Interests Single-molecule;Field-effect transistor;Atomic force microscopy;Surface chemistry;Bio-sensing
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Appears in Collections:
Division of Biomedical Technology 1. Journal Articles
Division of AI, Big data and Block chain Lab for Single-molecule Bioscience at Nano-interface 1. Journal Articles
Department of New Biology Molecular Epigenetics Lab 1. Journal Articles

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