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Two distinct domains of Flo8 activator mediates its role in transcriptional activation and the physical interaction with Mss11

Two distinct domains of Flo8 activator mediates its role in transcriptional activation and the physical interaction with Mss11
Kim, Hye YoungLee, Sung BaeKang, Hyen SamOh, Goo TaegKim, TaeSoo
DGIST Authors
Lee, Sung Bae
Issued Date
Article Type
Amino Terminal SequenceCell Surface ProteinDNA-BindingFLO1FLO11Flo8FLO8 GeneFungal GeneFungal ProteinGeneGene DeletionGene Expression Regulation, FungalGene TargetingGenes, FungalGlucan 1,4 Alpha GlucosidaseHeterodimerHomodimerLish MotifMannose-Binding LectinsMembrane GlycoproteinsMss11Mss11 ProteinMultiprotein ComplexesNon-HumanNuclear ProteinsPriority JournalProtein DomainProtein Interaction Domains and MotifsProtein MotifRecombinant Fusion ProteinsSaccharomyces CerevisiaeSaccharomyces Cerevisiae ProteinsTrans-ActivatorsTranscription FactorsTranscription InitiationTranscriptional ActivationUnclassified Drug
Flo8 is a transcriptional activator essential for the inducible expression of a set of target genes such as STA1, FLO11, and FLO1 encoding an extracellular glucoamylase and two cell surface proteins, respectively. However, the molecular mechanism of Flo8-mediated transcriptional activation remains largely elusive. By generating serial deletion constructs, we revealed here that a novel transcriptional activation domain on its extreme C-terminal region plays a crucial role in activating transcription. On the other hand, the N-terminal LisH motif of Flo8 appears to be required for its physical interaction with another transcriptional activator, Mss11, for their cooperative transcriptional regulation of the shared targets. Additionally, GST pull-down experiments uncovered that Flo8 and Mss11 can directly form either a heterodimer or a homodimer capable of binding to DNA, and we also showed that this formed complex of two activators interacts functionally and physically with the Swi/Snf complex. Collectively, our findings provide valuable clues for understanding the molecular mechanism of Flo8-mediated transcriptional control of multiple targets. © 2014 Elsevier Inc. All rights reserved.
Academic Press Inc.
Related Researcher
  • 이성배 Lee, Sung Bae 뇌과학과
  • Research Interests Cellular mechanism of neurodegenerative diseases; Neuronal maintenance and remodeling; 퇴행성 뇌질환의 세포기전; 신경계 유지 및 리모델링 연구
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Department of Brain Sciences Laboratory of Neurodegenerative Diseases and Aging 1. Journal Articles


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