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Soy soluble polysaccharide induces apoptosis in HCT-116 human colon cancer cells via reactive oxygen species generation

Soy soluble polysaccharide induces apoptosis in HCT-116 human colon cancer cells via reactive oxygen species generation
Ko, YJ[Ko, Yu-Jin]Jeong, JW[Jeong, Jin-Woo]Choi, YH[Choi, Yung-Hyun]Ryu, CH[Ryu, Chung-Ho]
DGIST Authors
Jeong, JW[Jeong, Jin-Woo]
Issued Date
Article Type
AcetylcysteineApoptosisCancer CellCancer InhibitionCaspaseCaspase 3CaspasesCell ProliferationCell ProteinCell Strain HCT116Cell StructureCell ViabilityChromosome CondensationColon CancerColon TumorColonic NeoplasmsConcentration ResponseControlled StudyCytochrome CCytochromes CDNA ContentDown-RegulationDrug EffectsEnzyme ActivationEnzyme ActivityEnzymologyHct116 Cell LineHct116 CellsHumanHuman CellHumansIncubation TimeMembrane Potential, MitochondrialMetabolismMitochondrial Membrane PotentialNicotinamide Adenine Dinucleotide Adenosine Diphosphate RibosyltransferasePathologyPolysaccharidePolysaccharidesProtein BADProtein BaxProtein BCL 2Protein BCL XLProtein ExpressionProto-Oncogene Proteins C-BCL-2Reactive Oxygen MetaboliteReactive Oxygen Species (ROS)Reactive Oxygen Species GenerationSoy SauceSoy Soluble PolysaccharideUnclassified DrugUpregulationX Linked Inhibitor of Apoptosis
Previous studies have suggested that soy sauce contains specific bioactive components and various biological activities of soy sauce have been observed. Soy soluble polysaccharide (SSPS), a predominant bioactive compound in soy sauce, has numerous pharmacological actions, including anti-inflammatory and immunomodulating activities. In the current study, the apoptotic effects of SSPS were investigated in HCT-116 human colon cancer cells. Treatment with SSPS significantly inhibited cell growth in a concentration-dependent manner by inducing apoptosis but not necrosis. This induction was associated with the generation of reactive oxygen species (ROS), mitochondrial dysfunction, activation of caspases and cleavage of the poly (ADP-ribose) polymerase protein. Induction of apoptotic cell death of HCT-116 cells by SSPS showed a correlation with the downregulation of members of the inhibitor of apoptosis protein family, including X-linked inhibitor of apoptosis protein and antiapoptotic Bcl-2, and upregulation of Bax and Bad. Administration of N-acetyl-L-cysteine, a scavenger of ROS, significantly decreased SSPS-induced apoptosis. These results indicate a critical role of signaling cascades involving a ROS-mediated caspase pathway in the anticancer effects of SSPS.
Spandidos Publications
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