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Stable Protein Device Platform Based on Pyridine Dicarboxylic Acid-Bound Cubic-Nanostructured Mesoporous Titania Films
- Stable Protein Device Platform Based on Pyridine Dicarboxylic Acid-Bound Cubic-Nanostructured Mesoporous Titania Films
- Kim, H[Kim, Hwajeong]; Park, SS[Park, Sung Soo]; Seo, J[Seo, Jooyeok]; Ha, CS[Ha, Chang-Sik]; Moon, C[Moon, Cheil]; Kim, Y[Kim, Youngkyoo]
- DGIST Authors
- Moon, C[Moon, Cheil]
- Issue Date
- ACS Applied Materials and Interfaces, 5(15), 6873-6878
- Article Type
- Biomedical Engineering; Carboxylic Acids; Chemistry; Convergence of Numerical Methods; Crystallization; Cytochrome C; Cytochromes C; Devices; Dicarboxylic Acid; Dicarboxylic Acids; Electrochemistry; Electron; Electrons; Equipment And Supplies; Esterification; Human; Humans; Mesoporous Materials; Mesoporous Titania; Metallic Films; Methodology; Molecules; Nano-Structures; Nanomaterial; Organic Coupler; Oxidation-Reduction; Oxidation-Reduction Reaction; Oxygen; Porosity; Procedures; Protein; Protein Device; Protein Devices; Proteins; Pyridine; Pyridine Derivative; Pyridines; Stability; Substrates; Superoxide; Superoxides; Surface Properties; Surface Property; Time; Time Factors; Titanium; Titanium Dioxide
- Here we shortly report a protein device platform that is extremely stable in a buffer condition similar to human bodies. The protein device platform was fabricated by covalently attaching cytochrome c (cyt c) protein molecules to organic coupler molecules (pyridine dicarboxylic acid, PDA) that were already covalently bound to an electron-transporting substrate. A cubic nanostructured mesoporous titania film was chosen as an electron-transporting substrate because of its large-sized cubic holes (∼7 nm) and highly crystalline cubic titania walls (∼0.4 nm lattice). Binding of PDA molecules to the mesoporous titania surface was achieved by esterification reaction between carboxylic acid groups (PDA) and hydroxyl groups (titania) in the presence of 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide (EDC) mediator, whereas the immobilization of cyt c to the PDA coupler was carried out by the EDC-mediated amidation reaction between carboxylic acid groups (PDA) and amine groups (cyt c). Results showed that the 2,4-position isomer among several PDAs exhibited the highest oxidation and reduction peak currents. The cyt c-immobilized PDA-bound titania substrates showed stable and durable electrochemical performances upon continuous current-voltage cycling for 240 times (the final current change was less than 3%) and could detect superoxide that is a core indicator for various diseases including cancers. © 2013 American Chemical Society.
- American Chemical Society
- Related Researcher
Brain convergent science based on chemical senses; olfaction; 감각신경계 기반 뇌융합과학; 후각 신경계
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- Department of Brain and Cognitive SciencesMoon Lab1. Journal Articles
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