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Stable Protein Device Platform Based on Pyridine Dicarboxylic Acid-Bound Cubic-Nanostructured Mesoporous Titania Films

Stable Protein Device Platform Based on Pyridine Dicarboxylic Acid-Bound Cubic-Nanostructured Mesoporous Titania Films
Kim, H[Kim, Hwajeong]Park, SS[Park, Sung Soo]Seo, J[Seo, Jooyeok]Ha, CS[Ha, Chang-Sik]Moon, C[Moon, Cheil]Kim, Y[Kim, Youngkyoo]
DGIST Authors
Moon, C[Moon, Cheil]
Issued Date
Article Type
Biomedical EngineeringCarboxylic AcidsChemistryConvergence of Numerical MethodsCrystallizationCytochrome CCytochromes CDevicesDicarboxylic AcidDicarboxylic AcidsElectrochemistryElectronElectronsEquipment And SuppliesEsterificationHumanHumansMesoporous MaterialsMesoporous TitaniaMetallic FilmsMethodologyMoleculesNano-StructuresNanomaterialOrganic CouplerOxidation-ReductionOxidation-Reduction ReactionOxygenPorosityProceduresProteinProtein DeviceProtein DevicesProteinsPyridinePyridine DerivativePyridinesStabilitySubstratesSuperoxideSuperoxidesSurface PropertiesSurface PropertyTimeTime FactorsTitaniumTitanium Dioxide
Here we shortly report a protein device platform that is extremely stable in a buffer condition similar to human bodies. The protein device platform was fabricated by covalently attaching cytochrome c (cyt c) protein molecules to organic coupler molecules (pyridine dicarboxylic acid, PDA) that were already covalently bound to an electron-transporting substrate. A cubic nanostructured mesoporous titania film was chosen as an electron-transporting substrate because of its large-sized cubic holes (∼7 nm) and highly crystalline cubic titania walls (∼0.4 nm lattice). Binding of PDA molecules to the mesoporous titania surface was achieved by esterification reaction between carboxylic acid groups (PDA) and hydroxyl groups (titania) in the presence of 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide (EDC) mediator, whereas the immobilization of cyt c to the PDA coupler was carried out by the EDC-mediated amidation reaction between carboxylic acid groups (PDA) and amine groups (cyt c). Results showed that the 2,4-position isomer among several PDAs exhibited the highest oxidation and reduction peak currents. The cyt c-immobilized PDA-bound titania substrates showed stable and durable electrochemical performances upon continuous current-voltage cycling for 240 times (the final current change was less than 3%) and could detect superoxide that is a core indicator for various diseases including cancers. © 2013 American Chemical Society.
American Chemical Society
Related Researcher
  • 문제일 Moon, Cheil 뇌과학과
  • Research Interests Brain convergent science based on chemical senses; olfaction; 감각신경계 기반 뇌융합과학; 후각 신경계
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Department of Brain Sciences Laboratory of Chemical Senses 1. Journal Articles


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