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Surface immobilization of MEPE peptide onto HA/ss-TCP ceramic particles enhances bone regeneration and remodeling

Surface immobilization of MEPE peptide onto HA/ss-TCP ceramic particles enhances bone regeneration and remodeling
Acharya, B[Acharya, Bodhraj]Chun, SY[Chun, So-Young]Kim, SY[Kim, Shin-Yoon]Moon, C[Moon, Cheil]Shin, HI[Shin, Hong-In]Park, EK[Park, Eui Kyun]
DGIST Authors
Moon, C[Moon, Cheil]
Issued Date
Article Type
Acid Phosphatase Tartrate Resistant IsoenzymeAnimal ExperimentAnimal ModelAnimalsBioactive PeptidesBoneBone AreaBone DefectBone MineralizationBone RegenerationBone RemodelingCalcium PhosphateCalcium Phosphate CeramicCalcium Phosphate CeramicsCalcium PhosphatesCalvariaCalvarial DefectsCarboxy Terminal SequenceCell DifferentiationCells, CulturedCeramic ParticleCovalent BondDisease Models, AnimalDurapatiteExtracellularExtracellular Matrix ProteinsFT-IRGlycoproteinsHematopoietic Stem CellHumanHuman CellHumansHydroxyapatiteImmobilized ProteinsIn-SituInfrared SpectroscopyMaleMatrix Extracellular PhosphoglycoproteinMEPE and Bone Marrow Stem CellMiceMice, Inbred ICRMicro-Computed TomographyMicro CTMouseNon-HumanOsteoblastOsteoblast DifferentiationOsteoblastsOsteoclastOsteoclastsOsteogenic PotentialPeptide ImmobilizationPeptidesPhosphatasesPhosphoproteinsProtein ImmobilizationScaffolds (Biology)Skull FracturesStem CellsSurface ImmobilizationSurface ModificationSurface TreatmentTri-Calcium PhosphatesX Ray Photoelectron Spectroscopy
Calcium phosphate ceramics have been widely used as scaffolds for bone regeneration. Here, to improve the osteogenic potential of hydroxyapatite/ β-tricalcium phosphate (HA/β-TCP) and to apply the bioactive peptide in situ, matrix extracellular phosphoglycoprotein (MEPE) peptide, which has been shown to stimulate osteoblast differentiation, was covalently and directionally immobilized on HA/β-TCP particles. The free-hydroxyl groups on the surface of the HA/β-TCP particles were sequentially conjugated with APTES, PEG-(SS) 2, and the synthetic MEPE peptide. Using FTIR and XPS, immobilization of the MEPE peptide on the HA/β-TCP was confirmed. Implantation of the MEPE peptide-immobilized HA/β-TCP into calvarial defect and subsequent analyses using a micro CT and histology showed significant bone regeneration and increased bone area (9.89-fold) as compared to that of unmodified HA/β-TCP. Moreover, tartrate-resistant acid phosphatase-positive osteoclasts were observed in regenerated bone by the MEPE peptide-immobilized HA/β-TCP, indicating that the bones newly formed by the MEPE peptide-immobilized HA/β-TCP are actively remodeled by osteoclasts. Therefore, our data demonstrate that MEPE peptide immobilization onto the HA/β-TCP surface stimulates bone regeneration associated with physiological bone remodeling. © 2012 WILEY PERIODICALS, INC.
Wiley Blackwell
Related Researcher
  • 문제일 Moon, Cheil 뇌과학과
  • Research Interests Brain convergent science based on chemical senses; olfaction; 감각신경계 기반 뇌융합과학; 후각 신경계
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Department of Brain Sciences Laboratory of Chemical Senses 1. Journal Articles


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