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Anti-inflammatory effects of 5-hydroxy-3,6,7,8,3 ',4 '-hexamethoxyflavone via NF-kappa B inactivation in lipopolysaccharide-stimulated RAW 264.7 macrophage

Anti-inflammatory effects of 5-hydroxy-3,6,7,8,3 ',4 '-hexamethoxyflavone via NF-kappa B inactivation in lipopolysaccharide-stimulated RAW 264.7 macrophage
Kim, MJ[Kim, Min Jeong]Lee, HH[Lee, Hye Hyeon]Jeong, JW[Jeong, Jin Woo]Seo, MJ[Seo, Min Jeong]Kang, BW[Kang, Byoung Won]Park, JU[Park, Jeong Uck]Kim, KS[Kim, Kyoung-Sook]Cho, YS[Cho, Young-Su]Seo, KI[Seo, Kwon-Il]Kim, GY[Kim, Gi-Young]Kim, JI[Kim, Jung-In]Choi, YH[Choi, Yung Hyun]Jeong, YK[Jeong, Yong Kee]
DGIST Authors
Jeong, JW[Jeong, Jin Woo]
Issued Date
Article Type
5-Hydroxy-3,6,7,8,3′,4′-Hexamethoxyflavone5 Hydroxy 3,6,7,8,3&apos,4&aposHexamethoxyflavoneAlgaAnimal CellAnimalsAnti-InflammatoryAnti-Inflammatory ActivityAnti-Inflammatory AgentAnti-Inflammatory AgentsCell CultureCell DeathCell LineCell NucleusCell SurvivalConfocal MicroscopyCyclooxygenase 2Cytokine ProductionCytosolCytotoxicityDinoprostoneDown-RegulationFlavone DerivativeFlavonesGene ExpressionGlyceraldehyde 3 Phosphate DehydrogenaseHizikia FusiformeI Kappa B AlphaImmunofluorescenceInducible Nitric Oxide SynthaseInterleukin-1 BetaLipopolysaccharideLipopolysaccharidesMacrophageMacrophagesMiceMTT AssayNF-Kappa BNitric OxideNitric Oxide Synthase Type IiiNon-Humannuclear factor-Kappa BProstaglandin E2Protective AgentsProtein DegradationReverse Transcriptase-Polymerase Chain ReactionSignal TransductionTranscription Factor RelaTumor Necrosis Factor-AlphaUnclassified DrugWestern Blotting
The anti-inflammatory mechanism of 5-hydroxy-3,6,7,8,3′,4′- hexamethoxyflavone (5HHMF), a polyhydroxyflavone isolated from the marine algae Hizikia fusiforme, was investigated in RAW 264.7 murine macrophage cells. Western blot and reverse transcriptase PCR analyses indicated that adding 5HHMF to cultured cells significantly reduced the production of nitric oxide and prostaglandin E2 and downregulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In addition, 5HHMF inhibited the release of pro-inflammatory cytokines, such as tumor necrosis factor-α and interleukin-1β, and decreased the transcriptional levels. In particular, 5HHMF significantly inhibited the LPS-induced nuclear factor-κB (NF-κB) translocation from the cytosol to the nucleus, which was associated with the abrogation of inhibitory IκBα degradation and subsequent decreases in nuclear p65 levels. In conclusion, these results suggested that the anti-inflammatory activities of 5HHMF may be attributed to the inhibition of iNOS, COX-2 and cytokine expression by attenuating NF-κB activation via IκBβ degradation in macrophages.
Spandidos Publications
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