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Improved neural progenitor cell proliferation and differentiation on poly(lactide-co-glycolide) scaffolds coated with elastin-like polypeptide
- Improved neural progenitor cell proliferation and differentiation on poly(lactide-co-glycolide) scaffolds coated with elastin-like polypeptide
- Choi, Seong-Kyoon; Park, Jin-Kyu; Lee, Kyeong-Min; Lee, Soo-Keun; Jeon, Won Bae
- DGIST Authors
- Lee, Kyeong-Min; Lee, Soo-Keun; Jeon, Won Bae
- Issue Date
- Journal of Biomedical Materials Research Part B: Applied Biomaterials, 101(8), 1329-1339
- Article Type
- 2' ,3' Cyclic Nucleotide 3' Phosphodiesterase; Amino Acid Motifs; Astrocyte; Astrocytes; Beta Tubulin; Biological Marker; Biological Markers; Biological Materials; Cell Adhesion; Cell Differentiation; Cell Lineage; Cell Proliferation; Chemical Structure; Chemistry; Complementary Dna; Concentration-Dependent Manners; Copolymers; Cytology; Elastin; Elastin-Like Polypeptide; Elastin-Like Polypeptides; Escherichia Coli; Extracellular Matrices; Extracellular Matrix; Fourier Transformation; Gene Expression; Gene Sequence; Glial Fibrillary Acidic Protein; Glyceraldehyde 3 Phosphate Dehydrogenase; Human; Human Cell; Humans; Infrared Spectroscopy; Messenger RNA; Metabolism; Microtubule Associated Protein 2; Morphology; Neural Progenitor Cells; Neural Stem Cell; Neural Stem Cells; Neural Tissue Engineering; Neuron Specific Enolase; Nucleotide Sequence; Peptide; Peptides; Phase Transition; PLGA Scaffolds; Poly-Lactide-Co-Glycolide; Polyglactin; Polyglactin 910; Polypeptide; Porosity; Procedures; Protein Motif; Real Time Polymerase Chain Reaction; Retinoic Acid; Reverse Transcriptase-Polymerase Chain Reaction; Scaffolds (Biology); Scanning Electron Microscopy; Spectroscopy, Fourier Transform Infrared; Stem Cells; Stem/Progenitor Cells; Surface Modification; Surface Property; Surface Treatment; Tissue Engineering; Tissue Engineering Applications; Tissue Scaffold; Tissue Scaffolds; Tretinoin; Unclassified Drug
- Poly(lactide-co-glycolide) (PLGA) and elastin-like polypeptide (ELP) have been widely used as a biodegradable scaffold and thermoresponsive matrix, respectively. However, little attention has focused on the combinatorial use of these biomaterials for tissue engineering applications. An ELP matrix TGPG[VGRGD(VGVPG)6]20WPC (referred to as REP) contains multiple Arg-Gly-Asp motifs. This study fabricated porous PLGA scaffolds coated with various concentration of matrix via thermally induced phase transition to improve adhesion-mediated proliferation and differentiation of neural progenitor cells. Matrix-coated scaffolds were characterized by FTIR, SEM, and hematoxylin and eosin staining with respect to coating efficiency, porosity, and pore size and shape. On the matrix-coated scaffolds, cells grew as a single cell or associated each other to form a multicellular layer or cluster. In biological evaluations, cell adhesion and proliferation were significantly promoted in a matrix concentration-dependent manner. More importantly, in combination with retinoic acid, the differentiation of progenitor cells into neuronal and astroglial lineages was highly stimulated in the cells cultured on matrix-coated scaffolds than on untreated controls. Taken together, our results indicated that the REP matrix-functionalized PLGA scaffolds are suitable for improving neuronal cell functions, and thus applicable for neural tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 101B: 1329-1339, 2013. Copyright © 2013 Wiley Periodicals, Inc.
- Wiley Blackwell
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