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Improved neural progenitor cell proliferation and differentiation on poly(lactide-co-glycolide) scaffolds coated with elastin-like polypeptide

Improved neural progenitor cell proliferation and differentiation on poly(lactide-co-glycolide) scaffolds coated with elastin-like polypeptide
Choi, Seong-KyoonPark, Jin-KyuLee, Kyeong-MinLee, Soo-KeunJeon, Won Bae
DGIST Authors
Lee, Kyeong-MinLee, Soo-KeunJeon, Won Bae
Issue Date
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 101(8), 1329-1339
Article Type
2&apos,3&aposCyclic Nucleotide 3&aposPhosphodiesteraseAmino Acid MotifsAstrocyteAstrocytesBeta TubulinBiological MarkerBiological MarkersBiological MaterialsCell AdhesionCell DifferentiationCell LineageCell ProliferationChemical StructureChemistryComplementary DnaConcentration-Dependent MannersCopolymersCytologyElastinElastin-Like PolypeptideElastin-Like PolypeptidesEscherichia ColiExtracellular MatricesExtracellular MatrixFourier TransformationGene ExpressionGene SequenceGlial Fibrillary Acidic ProteinGlyceraldehyde 3 Phosphate DehydrogenaseHumanHuman CellHumansInfrared SpectroscopyMessenger RNAMetabolismMicrotubule Associated Protein 2MorphologyNeural Progenitor CellsNeural Stem CellNeural Stem CellsNeural Tissue EngineeringNeuron Specific EnolaseNucleotide SequencePeptidePeptidesPhase TransitionPLGA ScaffoldsPoly-Lactide-Co-GlycolidePolyglactinPolyglactin 910PolypeptidePorosityProceduresProtein MotifReal Time Polymerase Chain ReactionRetinoic AcidReverse Transcriptase-Polymerase Chain ReactionScaffolds (Biology)Scanning Electron MicroscopySpectroscopy, Fourier Transform InfraredStem CellsStem/Progenitor CellsSurface ModificationSurface PropertySurface TreatmentTissue EngineeringTissue Engineering ApplicationsTissue ScaffoldTissue ScaffoldsTretinoinUnclassified Drug
Poly(lactide-co-glycolide) (PLGA) and elastin-like polypeptide (ELP) have been widely used as a biodegradable scaffold and thermoresponsive matrix, respectively. However, little attention has focused on the combinatorial use of these biomaterials for tissue engineering applications. An ELP matrix TGPG[VGRGD(VGVPG)6]20WPC (referred to as REP) contains multiple Arg-Gly-Asp motifs. This study fabricated porous PLGA scaffolds coated with various concentration of matrix via thermally induced phase transition to improve adhesion-mediated proliferation and differentiation of neural progenitor cells. Matrix-coated scaffolds were characterized by FTIR, SEM, and hematoxylin and eosin staining with respect to coating efficiency, porosity, and pore size and shape. On the matrix-coated scaffolds, cells grew as a single cell or associated each other to form a multicellular layer or cluster. In biological evaluations, cell adhesion and proliferation were significantly promoted in a matrix concentration-dependent manner. More importantly, in combination with retinoic acid, the differentiation of progenitor cells into neuronal and astroglial lineages was highly stimulated in the cells cultured on matrix-coated scaffolds than on untreated controls. Taken together, our results indicated that the REP matrix-functionalized PLGA scaffolds are suitable for improving neuronal cell functions, and thus applicable for neural tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 101B: 1329-1339, 2013. Copyright © 2013 Wiley Periodicals, Inc.
Wiley Blackwell
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