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Phosphoinositide and Erk signaling pathways mediate activity-driven rodent olfactory sensory neuronal survival and stress mitigation

Phosphoinositide and Erk signaling pathways mediate activity-driven rodent olfactory sensory neuronal survival and stress mitigation
Kim, So YeunMammen, AlexYoo, Seung JunCho, BongkiKim, Eun KyoungPark, Jong-InMoon, CheilRonnett, Gabriele V.
DGIST Authors
Kim, So Yeun; Mammen, Alex; Yoo, Seung Jun; Cho, Bongki; Kim, Eun Kyoung; Park, Jong-In; Moon, Cheil; Ronnett, Gabriele V.
Issue Date
Journal of Neurochemistry, 134(3), 486-498
Article Type
Animal TissueAnimalsBlotting, NorthernC57Bl MouseCell StressCell SurvivalCell SurvivalCell ViabilityControlled StudyCyclic AMPDual 2nd Messenger PathwaysEnzyme ActivationEnzyme ActivityEnzyme InhibitionEnzyme PhosphorylationHeat Shock Protein 70ImmunoblottingIn Vitro StudyIn Vivo StudyIntracellular SpaceIsovaleric AcidKinase PathwayLipid HydrolysisMaleMap Kinase Signaling SystemMiceMice, Inbred C57BLMitochondrial Membrane PotentialMitogen Activated Protein KinaseMitogen Activated Protein KinaseMitogen Activated Protein Kinase 1Mitogen Activated Protein Kinase 3Mitogen Activated Protein Kinase Kinase 1Mitogen Activated Protein Kinase Kinase 2MouseNerve Cell CultureNon HumanNorthern BlottingOdorOlfactoryOlfactory ReceptorOlfactory Receptor NeuronsOlfactory Sensory NeuronOlfactory StimulationOlfactory SystemOxidative StressOxidative StressOxidative StressPhorbol EsterPhosphatidylinositidePhosphatidylinositolPhosphatidylinositolsPhosphoinositidePhysiologyPriority JournalProtein ExpressionRatRat Hippocampal NeuronsRatsRats, Sprague DawleyReactive Oxygen MetaboliteReceptor NeuronsRodentiaSensory Nerve CellSensory StimulationSensory TransductionSignal TransductionSmall Interfering RnaSmellSprague Dawley RatSuperoxide ProductionTransgenic MiceUnilateral Naris OcclusionUpregulationActivity Driven SurvivalAdenovirus InfectionAdenovirus VectorAdenylyl CyclaseAdultAdult MiceAnimalAnimal CellAnimal ExperimentAnimal ModelImmunoblottingImmunohistochemistryImmunohistochemistry
Olfactory sensory neurons (OSNs) are the initial site for olfactory signal transduction. Therefore, their survival is essential to olfactory function. In the current study, we demonstrated that while odorant stimulation promoted rodent OSN survival, it induced generation of reactive oxygen species in a dose- and time-dependent manner as well as loss of membrane potential and fragmentation of mitochondria. The MEK-Erk pathway played a critical role in mediating these events, as its inhibition decreased odorant stimulation-dependent OSN survival and exacerbated intracellular stress measured by reactive oxygen species generation and heat-shock protein 70 expression. The phosphoinositide pathway, rather than the cyclic AMP pathway, mediated the odorant-induced activation of the MEK-Erk pathway. These findings provide important insights into the mechanisms of activity-driven OSN survival, the role of the phosphoinositide pathway in odorant signaling, and demonstrate that odorant detection and odorant stimulation-mediated survival proceed via independent signaling pathways. This mechanism, which permits independent regulation of odorant detection from survival signaling, may be advantageous if not diminished by repeated or prolonged odor exposure. © 2015 International Society for Neurochemistry.
Blackwell Publishing Inc.
Related Researcher
  • Author Moon, Cheil Laboratory of Chemical Senses
  • Research Interests Brain convergent science based on chemical senses; olfaction; 감각신경계 기반 뇌융합과학; 후각 신경계
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Division of Biotechnology1. Journal Articles
Department of Brain and Cognitive SciencesLab of Neuro-Metabolism & Neurometabolomic Research Center1. Journal Articles
Department of Brain and Cognitive SciencesLaboratory of Chemical Senses1. Journal Articles

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