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Abiotic synthesis of purine and pyrimidine ribonucleosides in aqueous microdroplets

Title
Abiotic synthesis of purine and pyrimidine ribonucleosides in aqueous microdroplets
Authors
Nam, In HoNam, Hong GilZare, Richard N
DGIST Authors
Nam, Hong Gil
Issue Date
2018-01
Citation
Proceedings of the National Academy of Sciences of the United States of America, 115(1), 36-40
Type
Article
Article Type
Article
Keywords
D-RIBOSE 1-PHOSPHATECHARGED MICRODROPLETSATMOSPHERIC AEROSOLSMASS-SPECTROMETRYORIGINLIFESALVAGEELECTROSPRAYINTERFACECHEMISTRY
ISSN
0027-8424
Abstract
Aqueous microdroplets (< 1.3 mu m in diameter on average) containing 15 mM D-ribose, 15 mM phosphoric acid, and 5 mM of a nucleobase (uracil, adenine, cytosine, or hypoxanthine) are electrosprayed from a capillary at + 5 kV into a mass spectrometer at room temperature and 1 atm pressure with 3 mM divalent magnesium ion (Mg2+) as a catalyst. Mass spectra show the formation of ribonucleosides that comprise a four-letter alphabet of RNA with a yield of 2.5% of uridine (U), 2.5% of adenosine (A), 0.7% of cytidine (C), and 1.7% of inosine (I) during the flight time of similar to 50 mu s. In the case of uridine, no catalyst is required. An aqueous solution containing guanine cannot be generated under the same conditions given the extreme insolubility of guanine in water. However, inosine can base pair with cytidine and thus substitute for guanosine. Thus, a full set of ribonucleosides to generate the purine-pyrimidine base pairs A-U and I-C are spontaneously generated in aqueous microdroplets under similar mild conditions.
URI
http://hdl.handle.net/20.500.11750/5600
DOI
10.1073/pnas.1718559115
Publisher
NATL ACAD SCIENCES
Related Researcher
  • Author Nam, Hong Gil CBRG(Complex Biology Research Group)
  • Research Interests Plant Aging and Life History; Systems Biology; Complexbiology; Comparative Aging Research
Files:
There are no files associated with this item.
Collection:
Department of New BiologyCBRG(Complex Biology Research Group)1. Journal Articles


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