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The C-Domain of the NAC Transcription Factor ANAC019 Is Necessary for pH-Tuned DNA Binding through a Histidine Switch in the N-Domain

Title
The C-Domain of the NAC Transcription Factor ANAC019 Is Necessary for pH-Tuned DNA Binding through a Histidine Switch in the N-Domain
Authors
Kang, Moo SeokKim, Sang YeolKim, Hyo JungShrestha, PraveshYun, Ji-HyePhee, Bong-KwanLee, WeontaeNam, Hong GilChang, Ik Soo
DGIST Authors
Nam, Hong GilChang, Ik Soo
Issue Date
2018-01
Citation
Cell Reports, 22(5), 1141-1150
Type
Article
Article Type
Article
Keywords
ARABIDOPSIS-THALIANASIGNAL-TRANSDUCTIONFACTOR FAMILYMEMBER
ISSN
2211-1247
Abstract
The affinity of transcription factors (TFs) for their target DNA is a critical determinant of gene expression. Whether the DNA-binding domain (DBD) of TFs alone can regulate binding affinity to DNA is an important question for identifying the design principle of TFs. We studied ANAC019, a member of the NAC TF family of proteins in Arabidopsis, and found a well-conserved histidine switch located in its DBD, which regulates both homodimerization and transcriptional control of the TF through H135 protonation. We found that the removal of a C-terminal intrinsically disordered region (IDR) in the TF abolished the pH-dependent binding of the N-terminal DBD to DNA. We propose a mechanism in which long-range electrostatic interactions between DNA and the negatively charged C-terminal IDR turns on the pH dependency of the DNA-binding affinity of the N-terminal DBD. Kang et al. find a histidine switch in the DNA-binding N-domain of the transcription factor ANAC019 that regulates both pH-dependent homodimerization and DNA binding. They propose that long-range electrostatic interactions between DNA and the negatively charged C-terminal turns on the pH dependency of the DNA-binding affinity of the N-terminal DNA-binding domain. © 2018 The Authors
URI
http://hdl.handle.net/20.500.11750/5883
DOI
10.1016/j.celrep.2018.01.002
Publisher
CELL PRESS
Related Researcher
  • Author Chang, Ik Soo Theoretical and Computational Biophysics Laboratory
  • Research Interests Theoretical and Computational Biophysics; Supercomputing Simulation of Biomolecules; 이론?계산 생물물리학; 통계물리학; 단백질체의 슈퍼컴퓨터 모델링 및 시물레이션
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Collection:
Department of New BiologyCBRG(Complex Biology Research Group)1. Journal Articles
Department of Brain and Cognitive SciencesTheoretical and Computational Biophysics Laboratory1. Journal Articles


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