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Quantification of Autophagy During Senescence

Title
Quantification of Autophagy During Senescence
Authors
Park, Joon TaeLee, Young-SamPark, Sang Chul
DGIST Authors
Lee, Young-Sam
Issue Date
2019
Citation
Methods in molecular biology (Clifton, N.J.), 1896, 149-157
Type
Article
Article Type
Article
Keywords
Autophagic fluxAutophagyCYTO-ID® green dyeDQ™ Red BSASenescence
ISSN
1940-6029
Abstract
Autophagy constitutes an evolutionarily conserved catabolic process that contributes to the clearance of damaged cellular components in response to a variety of stress conditions. Additionally, it plays a variety of physiological and pathophysiological roles in maintaining cell homeostasis. Recently, the critical role of autophagy during cellular senescence has been supported by evidences demonstrating the reversal of senescence by the reestablishment of autophagy. As considerable attention has been directed toward understanding the molecular mechanisms underlying senescence and autophagy, a method to accurately quantify autophagy during senescence is critical to understand its role in senescence and senescence-related diseases. In this chapter, we describe the use of CYTO-ID® green dye and DQ™ Red BSA to monitor the autophagic flux as an accurate method to quantify autophagic activity. This technique relies on the specificity of CYTO-ID® green dye in staining autophagosome and the cleavage of the self-quenched DQ™ Red BSA protease substrates in an acidic compartment. In particular, herein we describe protocols to quantify autophagy during senescence.
URI
http://hdl.handle.net/20.500.11750/9490
DOI
10.1007/978-1-4939-8931-7_14
Publisher
NLM (Medline)
Related Researcher
  • Author Lee, Young-Sam Lab of genome maintenance
  • Research Interests DNA replication and repair; Restoration of cellular senescence; Structural and functional relationship of proteins
Files:
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Collection:
Department of New BiologyLab of Genome Maintenance1. Journal Articles


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