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Selective Activation of hTRPV1 by N-Geranyl Cyclopropylcarboxamide, an Amiloride-Insensitive Salt Taste Enhancer

Selective Activation of hTRPV1 by N-Geranyl Cyclopropylcarboxamide, an Amiloride-Insensitive Salt Taste Enhancer
Kim, Min JungSon, Hee JinKim, YiseulKweon, Hae JinSuh, Byung ChangLyall, VijayRhyu, Mee-Ra
DGIST Authors
Suh, Byung Chang
Issued Date
Article Type
Acid-Sensing Ion ChannelAcid-Sensing Ion ChannelsAmideAmidesAmilorideASIC1 Protein, HumanBenzamilCalcium ChannelCalcium ChannelsCalcium TransportCapsazepineCell CultureChemical StructureConcentration ResponseControlled StudyDrug EffectsEpithelial Sodium ChannelEpithelial Sodium ChannelsGenetic TransfectionGeneticsHEK293 Cell LineHEK293 CellsHumanHuman CellHumansMembrane PotentialMembrane PotentialsMetabolic ActivationMetabolismMonoterpenesN-Geranyl CyclopropylcarboxamideN GeranylcyclopropylcarboxamideNerve ProteinNerve Tissue ProteinsNucleotide SequencePharmacologyPhysiologyRuthenium RedSalt IntakeSensory NerveSignal TransductionSodium Chloride, DietarySodium TransportTasteTaste BudTaste BudsTaste PerceptionTerpeneTransfectionTransient Receptor Potential Channel (TRP Channel)Transient Receptor Potential ChannelsTRPA1 Protein, HumanTRPV Cation ChannelsTRPV1 Protein, HumanUnclassified DrugVanilloid ReceptorVanilloid Receptor1Vanilloid Receptor1 Antagonist
TRPV1t, a variant of the transient receptor potential vanilloid-1 (TRPV1) has been proposed as a constitutively active, non-selective cation channel as a putative amiloride-insensitive salt taste receptor and shares many properties with TRPV1. Based on our previous chorda tympani taste nerve recordings in rodents and human sensory evaluations, we proposed that N- geranylcyclopropylcarboxamide (NGCC), a novel synthetic compound, acts as a salt taste enhancer by modulating the amiloride/benzamil-insensitive Na+ entry pathways. As an extension of this work, we investigated NGCC-induced human TRPV1 (hTRPV1) activation using a Ca2+-flux signaling assay in cultured cells. NGCC enhanced Ca2+ influx in hTRPV1-expressing cells in a dose-dependent manner (EC50 = 115 μM). NGCC-induced Ca 2+ influx was significantly attenuated by ruthenium red (RR; 30 mM), a non-specific blocker of TRP channels and capsazepine (CZP; 5 μM), a specific antagonist of TRPV1, implying that NGCC directly activates hTRPV1. TRPA1 is often co-expressed with TRPV1 in sensory neurons. Therefore, we also investigated the effects of NGCC on hTRPA1-expressing cells. Similar to hTRPV1, NGCC enhanced Ca2+ influx in hTRPA1-expressing cells (EC50 = 83.65 μM). The NGCC-induced Ca2+ influx in hTRPA1-expressing cells was blocked by RR (30 μM) and HC-030031 (100 μM), a specific antagonist of TRPA1. These results suggested that NGCC selectively activates TRPV1 and TRPA1 in cultured cells. These data may provide additional support for our previous hypothesis that NGCC interacts with TRPV1 variant cation channel, a putative amiloride/benzamil-insensitive salt taste pathway in the anterior taste receptive field. © 2014 Kim et al.
Public Library of Science
Related Researcher
  • 서병창 Suh, Byung-Chang 뇌과학과
  • Research Interests Molecular mechanisms of epilepsy and sensory pain transmission; Signaling mechanism of ion channel regulation and membrane excitability; 분자전기생리; 간질 및 통증의 분자적 기전 연구
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Department of Brain Sciences Laboratory of Brain Signal and Synapse Research 1. Journal Articles


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