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Regulation of autophagic cell death by glycogen synthase kinase-3 beta in adult hippocampal neural stem cells following insulin withdrawal

Title
Regulation of autophagic cell death by glycogen synthase kinase-3 beta in adult hippocampal neural stem cells following insulin withdrawal
Author(s)
Ha, ShinwonRyu, Hye YoungChung, Kyung MinBaek, Seung-HoonKim, Eun-KyoungYu, Seong-Woon
Issued Date
2015-05
Citation
Molecular Brain, v.8, no.1
Type
Article
Author Keywords
ApoptosisAutophagic cell deathGlycogen synthase kinase-3βHippocampal neural stem cellsProgrammed cell death
Keywords
ACTIVATIONAdultAnimal CellAnimal TissueAPOPTOSISArticleAutophagic Cell DeathAutophagosomeAutophagyBETA-CATENINBeta CateninCaspase 3Cell DeathCell ViabilityEnzyme ActivationEnzyme InactivationEXPRESSIONGene InactivationGene SilencingGlycogen Synthase Kinase-3 BetaGlycogen Synthase Kinase 3 AlphaGSK-3 BetaGSK3Hippocampal Neural stem CellHippocampal Neural stem CellsHippocampusINACTIVATIONINHIBITIONInsulinKINASELipocortin 5LysosomeMammal CellMammaliaNeural Stem CellNonhumanPharmacological BlockingPhosphorylationPriority JournalProgrammed Cell DeathPROTEINProtein ExpressionRatRattus
ISSN
1756-6606
Abstract
Background: Neural stem cells (NSCs) hold great potential for the treatment of neurodegenerative diseases. However, programmed cell death (PCD) provoked by the harsh conditions evident in the diseased brain greatly undermines the potential of NSCs. Currently, the mechanisms of PCD that effect NSCs remain largely unknown. Results: We have previously reported that hippocampal neural stem (HCN) cells derived from the adult rat brain undergo autopahgic cell death (ACD) following insulin withdrawal without hallmarks of apoptosis despite their normal apoptotic capabilities. In this study, we demonstrate that glycogen synthase kinase 3β (GSK-3β) induces ACD in insulin-deprived HCN cells. Both pharmacological and genetic inactivation of GSK-3β significantly decreased ACD, while activation of GSK-3β increased autophagic flux and caused more cell death without inducing apoptosis following insulin withdrawal. In contrast, knockdown of GSK-3α barely affected ACD, lending further support to the critical role of GSK-3β. Conclusion: Collectively, these data demonstrate that GSK-3β is a key regulator of ACD in HCN cells following insulin withdrawal. The absence of apoptotic indices in GSK-3β-induced cell death in insulin-deprived HCN cells corroborates the notion that HCN cell death following insulin withdrawal represents the genuine model of ACD in apoptosis-intact mammalian cells and identifies GSK-3β as a key negative effector of NSC survival downstream of insulin signaling. © 2015 Ha et al.; licensee BioMed Central.
URI
http://hdl.handle.net/20.500.11750/2898
DOI
10.1186/s13041-015-0119-9
Publisher
BioMed Central Ltd.
Related Researcher
  • 김은경 Kim, Eun-Kyoung
  • Research Interests Neural functions in metabolic diseases; 뇌신경세포와 비만; 당뇨 등의 대사 질환 관련 연구
Files in This Item:
10.1186_s13041_015_0119_9.pdf

10.1186_s13041_015_0119_9.pdf

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Appears in Collections:
Department of Brain Sciences Lab of Neuro-Metabolism & Neurometabolomic Research Center 1. Journal Articles
Department of Brain Sciences Laboratory of Neuronal Cell Death 1. Journal Articles

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