Cited 0 time in webofscience Cited 9 time in scopus

Hemodynamic monitoring of Chlorin e6-mediated photodynamic therapy using diffuse optical measurements

Hemodynamic monitoring of Chlorin e6-mediated photodynamic therapy using diffuse optical measurements
Dong, JingToh, Hui JinThong, Patricia S. P.Tee, Chuan SiaBi, RenzheSoo, Khee-CheeLee, Kijoon
DGIST Authors
Lee, Kijoon
Issue Date
Journal of Photochemistry and Photobiology B: Biology, 140, 163-172
Article Type
AngiogenesisAnimalAnimal CellAnimal ExperimentAnimal ModelAnimal TissueAnimalsAntineoplastic ActivityBagg Albino MouseBlood CirculationBlood FlowBlood Vessel ReactivityBreast TumorCell Line, TumorChlorin E6CirculationControlled StudyDeoxyhemoglobinDiffuse Correlation Spectroscopy (DCS)Diffuse Optical Spectroscopy (DOS)Diffusion CoefficientDose-ResponseDose-Response Relationship, DrugDrug Dose ComparisonDrug EffectsDrug MegadoseFrequency Domain Diffuse Optical SpectroscopyHemodynamic MonitoringHemodynamic ParametersHemodynamicsHemoglobinHemoglobin DeterminationLight Related PhenomenaLow Drug DoseMaleMammary Neoplasms, ExperimentalMetabolic RateMetabolismMiceMice, Inbred BALB CMouseNon-HumanOptical ProcessesOxygenOxygen SaturationOxyhemoglobinPathophysiologyPhotochemotherapyPhotodynamic Therapy (PDT)Photosensitizing AgentPhotosensitizing AgentsPorphyrinPorphyrinsPriority JournalReactive Oxygen MetaboliteRelative Blood Flow (RBF)Relative Tumor Metabolic Rate of OxygenReoxygenationSpectroscopySpectrum AnalysisStatistical SignificanceTimeTime FactorsTissue OxygenationTissue Oxygenation (StO2)Treatment OutcomeTreatment ResponseTumor Blood FlowTumor Cell LineTumor Metabolic Rate of Oxygen (TMRO2)Tumor VolumeTumor XenograftUnclassified Drug
Tumor response during photodynamic therapy (PDT) is heavily dependent on treatment parameters such as light dose, photosensitizer concentration, and tissue oxygenation. Therefore, it is desirable to have a real-time hemodynamic monitoring device in order to fine-tune the parameters and improve PDT efficacy. In this paper, such a tumor response monitoring system was built incorporating both frequency domain diffuse optical spectroscopy (FD-DOS) and diffuse correlation spectroscopy (DCS), which enables concurrent monitoring of tissue oxygenation (StO2), total hemoglobin concentration (THC) and relative blood flow (rBF). The tumor metabolic rate of oxygen (TMRO2) was calculated by using the hemodynamic parameters. Mouse models bearing xenograft tumors were subjected to chlorin e6 (Ce6)-mediated PDT, and the four parameters were monitored with varying treatment conditions. The results show (1) At 3 h post-PDT, rStO2, rBF and rTMRO2 exhibited sharp PDT-induced decreases in responders (>40% reduction in tumor volume). Statistically significant difference between responders and non-responders were observed in rStO2 and rBF, but not in rTMRO2. (2) Non-responders show gradual recovery of rStO2, rBF and rTMRO 2 from ∼24 h post-PDT, while responder group did not show recovery up until 48 h post-PDT. Long-term study results up to 2 weeks are also shown. It suggests the hybrid diffuse optical system is not only capable of real-time treatment monitoring, but also able to extract tumor metabolic rate of oxygen to provide more insights about therapy mechanism. Translation of this technique to the clinic will make a quick prognosis feasible and help with treatment optimization. © 2014 Elsevier B.V. All rights reserved.
Related Researcher
  • Author    
  • Research Interests
There are no files associated with this item.
School of Undergraduate Studies1. Journal Articles

qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.