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Gene regulatory cascade of senescence-associated NAC transcription factors activated by ETHYLENE-INSENSITIVE2-mediated leaf senescence signalling in Arabidopsis
- Gene regulatory cascade of senescence-associated NAC transcription factors activated by ETHYLENE-INSENSITIVE2-mediated leaf senescence signalling in Arabidopsis
- Kim, Hyo Jung; Hong, Sung Hyun; Kim, You Wang; Lee, Il Hwan; Jun, Ji Hyung; Phee, Bong-Kwan; Rupak, Timilsina; Jeong, Hana; Lee, Yeonmi; Hong, Byoung Seok; Nam, Hong Gil; Woo, Hye Ryun; Lim, Pyung Ok
- DGIST Authors
- Nam, Hong Gil; Woo, Hye Ryun; Lim, Pyung Ok
- Issue Date
- Journal of Experimental Botany, 65(14), 4023-4036
- Article Type
- Arabidopsis; Arabidopsis Protein; Arabidopsis Proteins; Biological Model; Cell Surface Receptor; EIN2-Mediated Senescence Signalling; EIN2 Protein, Arabidopsis; EIN3; Gene; Gene Expression Regulation; Gene Expression Regulation, Plant; Gene Regulatory Network; Gene Regulatory Networks; Genes, Plant; Genetics; Growth, Development and Aging; Metabolism; Models, Biological; Mutation; NAC Transcription Factor; Plant Gene; Plant Leaf; Plant Leaves; Promoter Region; Promoter Regions, Genetic; Protein Binding; Receptors, Cell Surface; Signal Transduction; Transcription Factor; Transcription Factors
- Leaf senescence is a finely tuned and genetically programmed degeneration process, which is critical to maximize plant fitness by remobilizing nutrients from senescing leaves to newly developing organs. Leaf senescence is a complex process that is driven by extensive reprogramming of global gene expression in a highly coordinated manner. Understanding how gene regulatory networks involved in controlling leaf senescence are organized and operated is essential to decipher the mechanisms of leaf senescence. It was previously reported that the trifurcate feed-forward pathway involving EIN2, ORE1, and miR164 in Arabidopsis regulates age-dependent leaf senescence and cell death. Here, new components of this pathway have been identified, which enhances knowledge of the gene regulatory networks governing leaf senescence. Comparative gene expression analysis revealed six senescence-associated NAC transcription factors (TFs) (ANAC019, AtNAP, ANAC047, ANAC055, ORS1, and ORE1) as candidate downstream components of ETHYLENE-INSENSITIVE2 (EIN2). EIN3, a downstream signalling molecule of EIN2, directly bound the ORE1 and AtNAP promoters and induced their transcription. This suggests that EIN3 positively regulates leaf senescence by activating ORE1 and AtNAP, previously reported as key regulators of leaf senescence. Genetic and gene expression analyses in the ore1 atnap double mutant revealed that ORE1 and AtNAP act in distinct and overlapping signalling pathways. Transient transactivation assays further demonstrated that ORE1 and AtNAP could activate common as well as differential NAC TF targets. Collectively, the data provide insight into an EIN2-mediated senescence signalling pathway that coordinates global gene expression during leaf senescence via a gene regulatory network involving EIN3 and senescence- associated NAC TFs. © 2014 The Author.
- Oxford University Press
- Related Researcher
Lim, Pyung Ok
CBRG(Complex Biology Research Group)
Aging and programmen cell death in plants; RNA-based regulation of leaf aging; Synthetic genetics-based aging reprogramming
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- Department of New BiologyCBRG(Complex Biology Research Group)1. Journal Articles
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