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A Microfluidic System for Investigating Anticipatory Medication Effects on Dopamine Homeostasis in Dopaminergic Cells

Title
A Microfluidic System for Investigating Anticipatory Medication Effects on Dopamine Homeostasis in Dopaminergic Cells
Author(s)
Kim, SamhwanKang, SeongtakChoe, JiyunMoon, CheilChoi, HongsooKim, Jin-YoungChoi, Ji-Woong
Issued Date
ACCEPT
Citation
Analytical Chemistry
Type
Article
ISSN
0003-2700
Abstract
Dopamine (DA) homeostasis influences emotions, neural circuit development, cognition, and the reward system. Dysfunctions in DA regulation can lead to neurological disorders, including depression, developmental disorders, and addiction. DA homeostasis disruption is a primary cause of Parkinson’s Disease (PD). Therefore, understanding the relationship between DA homeostasis and PD progression may clarify the mechanisms for pharmacologically treating PD. This study developed a novel in vitro DA homeostasis platform which consists of three main parts: (1) a microfluidic device for culturing DAergic neurons, (2) an optical detection system for reading DA levels, and (3) an automatic closed-loop control system that establishes when and how much medication to infuse; this uses a microfluidic device that can cultivate DAergic neurons, perfuse solutions, perform in vitro PD modeling, and continuously monitor DA concentrations. The automatically controlled closed-loop control system simultaneously monitors pharmacological PD treatment to support long-term monitoring of DA homeostasis. SH-SY5Y neuroblastoma cells were chosen as DAergic neurons. They were cultivated in the microfluidic device, and real-time cellular DA level measurements successfully achieved long-term monitoring and modulation of DA homeostasis. When applied in combination with multiday cell culture, this advanced system can be used for drug screening and fundamental biological studies.
URI
http://hdl.handle.net/20.500.11750/17505
DOI
10.1021/acs.analchem.2c04923
Publisher
American Chemical Society
Related Researcher
  • 문제일 Moon, Cheil 뇌과학과
  • Research Interests Brain convergent science based on chemical senses; olfaction; 감각신경계 기반 뇌융합과학; 후각 신경계
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Appears in Collections:
Department of Brain Sciences Laboratory of Chemical Senses 1. Journal Articles
Department of Robotics and Mechatronics Engineering Bio-Micro Robotics Lab 1. Journal Articles
Division of Biotechnology 1. Journal Articles
Department of Electrical Engineering and Computer Science CSP(Communication and Signal Processing) Lab 1. Journal Articles

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