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Spheroid Culture of Mammalian Olfactory Receptor Neurons: Potential Applications for a Bioelectronic Nose

Title
Spheroid Culture of Mammalian Olfactory Receptor Neurons: Potential Applications for a Bioelectronic Nose
Authors
Kim, Sam HwanKim, So YeunChoi Seong-KyunBae, JisubJeon, Won BaeJang, Jae EunMoon, Cheil
DGIST Authors
Choi Seong-Kyun; Jeon, Won BaeJang, Jae EunMoon, Cheil
Issue Date
2018-12
Citation
Experimental Neurobiology, 26(7), 574-598
Type
Article
Article Type
Article
Author Keywords
Cell-based biosensorRecombinant proteinOlfactory receptor neuronSpheroid cultureMicro electrode array
Keywords
SENSORY NEURONSACTIVATIONPROTEINDISCRIMINATIONSURVIVALSIGNALSSYSTEMSMELL
ISSN
1226-2560
Abstract
The olfactory system can detect many odorants with high sensitivity and selectivity based on the expression of nearly a thousand types of olfactory receptors (ORs) in olfactory receptor neurons (ORNs). These ORs have a dynamic odorant detection range and contribute to signal encoding processes in the olfactory bulb (OB). To harness the capabilities of the olfactory system and develop a biomimetic sensor, stable culture and maintenance of ORNs are required. However,in vitro monolayer culture models have several key limitations: i) short available period of cultured neurons, ii) low cultural efficiency, and iii) long-term storage challenges. This study aims to develop a technique: i) to support the spheroid culture of primary ORN precursors facilitating stable maintenance and long-term storage, and ii) to demonstrate the viability of ORN spheroid culture in developing an olfactory system mimetic bioelectronic nose. Recombinant protein (REP; TGPG[VGRGD(VGVPG)6]20WPC) was used to form the ORN spheroids. Spheroid formation enabled preservation of primary cultured ORNs without a significant decrease in viability or the expression of stemness markers for ten days. Physiological characteristics of the ORNs were verified by monitoring intracellular calcium concentration upon odorant mixture stimulation; response upon odorant stimulation were observed at least for ten days in these cultivated ORNs differentiated from spheroids. Coupling ORNs with multi electrode array (MEA) enabled the detection and discrimination of odorants by analyzing the electrical signal patterns generated following odorant stimulation. Taken together, the ORN spheroid culture process is a promising technique for the development of a bioelectronic nose and high-throughput odorant screening device. Copyright © Experimental Neurobiology 2018.
URI
http://hdl.handle.net/20.500.11750/9444
DOI
10.5607/en.2018.27.6.574
Publisher
한국뇌신경과학회
Related Researcher
Files:
There are no files associated with this item.
Collection:
Division of Biotechnology1. Journal Articles
Department of Information and Communication EngineeringAdvanced Electronic Devices Research Group(AEDRG) - Jang Lab.1. Journal Articles
Department of Brain and Cognitive SciencesLaboratory of Chemical Senses1. Journal Articles


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